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Status
Public on May 21, 2004
Title
B chip Version1: time 30 min Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1231
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
Reference key to the corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
1
203.127
244.989
226.113
201.912
124.5
183
157
134.5
2
-9.48E-02
0
251.328
228.1
223.39
190.965
152
162
159
119
3
1.89E-01
0
1166.1
240.027
1132.41
243.625
988
154
899
159
4
-1.46E+00
0
241.601
257.706
229.572
212.254
153
210
157
118
5
8.79E-02
0
814.658
241.793
795.12
211.917
716
159
662
138
6
1.92E-01
0
488.25
234.933
495.087
227.384
386
159
385
165
7
1.11E+00
0
287.701
228.162
223.825
219.347
198
166.5
155
160.5
8
2.54E-01
0
307.963
248.414
277.79
221.949
221
164
211
149.5
9
3.53E-02
0
399.583
247.295
392.461
224.132
305
175
295
147
10
-3.96E-01
0
263.644
251.563
249.418
204.904
198
173
183
124
11
1.06E-01
0
268.428
235.484
260.219
221.214
190.5
150.5
181.5
147.5
12
2.74E-01
0
270.051
244.77
234.3
227.145
211
180
160
150
13
8.00E-01
0
295.927
259.18
238.381
222.776
206
185
165
167
14
2.59E-01
0
261.874
217.396
230.587
199.608
185
144
154
128.5
15
1.84E-01
0
402.984
221.731
377.862
209.908
326
150
252
126
16
-9.10E-01
0
258.144
252.753
279.785
206.62
178
193.5
199
145
17
1.38E-01
0
423.344
230.028
386.182
182.381
337
163
312
117.5
18
-3.95E-01
0
250.974
232.971
224.638
195.463
204
160
146
99
19
6.51E-01
0
267.066
208.656
228.836
198.248
181
119
152
132
20
-2.04E-01
0
249.495
239.99
242.45
202.337
186
170
159
140
Total number of rows: 14720 Table truncated, full table size 947 Kbytes .Supplementary data files not provided