|
| Status |
Public on Sep 14, 2007 |
| Title |
AFFY-S52-A |
| Sample type |
RNA |
| |
|
| Source name |
Leaves, 52 hr after salicylic acid treatment, First Replica
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Plants were grown in soil in a growth chamber at 20 degrees C with 8 hours of light for 46 days. Half-strength Hoagland's solution was used to bottom-water the plants. Plants were sprayed to run-off with 0.3 mM SA in 0.02% silwet in the light. Fifty-two hours post-treatment, rosettes were cut above the soil line and harvested (in the light), quick-frozen in liquid nitrogen, then stored at -80 degrees C. Total RNA was later extracted using TRIzol reagent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction (Invitrogen), followed by RNeasy purification (Qiagen)
|
| Label |
as per GeneChip Expression Analysis Technical Manual 701021 rev1
|
| Label protocol |
started with 12 μg total RNA; biotin labeling using ENZO kit
|
| |
|
| Hybridization protocol |
as per GeneChip Expression Analysis Technical Manual 701021 rev1
|
| Scan protocol |
Affymetrix GeneArray 2500
|
| Description |
RNA extraction in Meyers Lab (http://mpss.udel.edu/). Affymetrix Experiment in St. Clair Lab (http://stclairlab.ucdavis.edu/).
|
| Data processing |
MAS 5.0 software was used with Absence Calls calculated.
|
| |
|
| Submission date |
Sep 07, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Junfeng Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Manchester
|
| Department |
School of Computer Science
|
| Street address |
Kilburn Building, Oxford Road
|
| City |
Manchester |
| ZIP/Postal code |
M13 9PL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE8994 |
A Comparison of microarray and MPSS Technology Platforms for Expression Analysis of Arabidopsis |
|
| Relations |
| Reanalyzed by |
GSE119083 |
