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Sample GSM22757 Query DataSets for GSM22757
Status Public on May 21, 2004
Title A chip: time 15 day Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 15 day Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -2.08E-01 0 89.306 56.2422 109.947 76.5994 17 8.5 35 15
2 3.62E-01 0 97.5137 67.6755 117.226 128.785 20 8 37 48
3 4.50E-01 0 393.94 65.125 382.142 90.6818 249 9 266 18.5
4 NULL 0 73.7632 63.159 127.082 96.4664 11 8 37 31
5 -1.75E-01 0 1157.19 97.6398 1750.34 112.516 962 27 1513 26.5
6 -8.82E-02 0 91.0196 65.4555 94.9762 43.9267 14 9 20 7
7 -3.91E-01 0 80.0929 42.6516 106.919 64.3402 12 7 27.5 9
8 -4.44E-01 0 57.8018 60.5 72.9895 52.2222 8 12 10.5 9.5
9 -9.83E-02 0 659.313 68.6468 973.375 77.1055 555 12 862 16.5
10 -1.18E-01 0 64.2896 55.8683 71.2871 64.0356 10 7 12 8
11 -6.88E-01 0 148.945 52.5502 300.128 61.1566 65 5 185 8
12 -4.12E-02 0 75.2928 45.2926 80.4685 61.6667 9 7 10 10
13 -2.27E-01 0 110.497 49.6699 143.829 46.8835 18 6 38 8
14 -3.45E-01 0 49.6539 49.7282 64.1985 71.2395 7 7 9 13
15 -2.31E-01 0 57.2576 50.0817 68.2671 45.4281 7 6 10 7
16 6.38E-02 0 72.3633 50.3911 71.7466 62.3026 11 7 11 9
17 3.29E-01 0 190.651 54.2297 183.012 76.5068 78 7 66.5 14
18 8.78E-02 0 283.149 59.2449 340.612 49.1293 162 7 191 7.5
19 -1.33E-01 0 61.3024 60.2362 64.9654 49.5566 8 9 10 7
20 -2.13E-02 0 70.7286 42.8867 75.0929 63.0906 10 7 17 10

Total number of rows: 16928

Table truncated, full table size 1020 Kbytes.




Supplementary data files not provided
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