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Sample GSM22754 Query DataSets for GSM22754
Status Public on May 21, 2004
Title A chip: time 5 day Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -1.13E-01 0 407.672 207.039 538.22 312.249 309 150 455 253.5
2 -1.18E+00 0 235.207 221.485 620.233 565.474 155.5 139 546.5 414
3 -5.50E-01 0 758.9 165.36 1188.68 289.935 662 97 945 207
4 -5.99E-01 0 246.312 199.785 262.228 166.689 180 133.5 181.5 97
5 -3.75E-01 0 4303.12 298.95 4743.14 204.524 3914 235 4382 133.5
6 -5.71E-02 0 504.75 195.542 519.543 180.355 413 110 417 99.5
7 -1.06E-01 0 271.117 181.156 237.34 144.364 211 108 148 63.5
8 1.72E-01 0 189.413 192.563 149.994 146.013 117 99 73 61
9 -2.28E-01 0 1556.52 196.127 1695.22 159.011 1181 165 1246 82
10 5.53E-01 0 255.461 193.154 183.781 161.937 165.5 128.5 94.5 91.5
11 -2.27E-01 0 330.24 163.155 331.795 129.173 259 95 249.5 51
12 -3.62E-01 0 320.042 195.901 333.064 159.778 252 126.5 233 79
13 -7.13E-01 0 1533.78 179.705 2354.72 166.809 1459 98 2307 85
14 1.36E+00 0 229.661 183.158 131.155 155.087 144 101 63 73
15 -5.44E-01 0 251.471 217.185 180.701 114.732 187.5 154.5 110.5 39.5
16 1.14E-01 0 222.754 166.871 168.123 128.378 151 111 91 60
17 3.55E-01 0 604.697 208.368 435.949 118.569 527.5 133 296.5 53.5
18 -1.19E-01 0 1648.57 247.708 1666.18 161.175 1555 178 1489 81.5
19 3.21E-01 0 253.569 200.91 187.004 155.437 170 130 102.5 84
20 -3.56E-01 0 265.177 198.369 208.142 135.256 187 148 119 61

Total number of rows: 16928

Table truncated, full table size 1089 Kbytes.




Supplementary data files not provided

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