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Status
Public on May 21, 2004
Title
A chip: time 5 day Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-1.13E-01
0
407.672
207.039
538.22
312.249
309
150
455
253.5
2
-1.18E+00
0
235.207
221.485
620.233
565.474
155.5
139
546.5
414
3
-5.50E-01
0
758.9
165.36
1188.68
289.935
662
97
945
207
4
-5.99E-01
0
246.312
199.785
262.228
166.689
180
133.5
181.5
97
5
-3.75E-01
0
4303.12
298.95
4743.14
204.524
3914
235
4382
133.5
6
-5.71E-02
0
504.75
195.542
519.543
180.355
413
110
417
99.5
7
-1.06E-01
0
271.117
181.156
237.34
144.364
211
108
148
63.5
8
1.72E-01
0
189.413
192.563
149.994
146.013
117
99
73
61
9
-2.28E-01
0
1556.52
196.127
1695.22
159.011
1181
165
1246
82
10
5.53E-01
0
255.461
193.154
183.781
161.937
165.5
128.5
94.5
91.5
11
-2.27E-01
0
330.24
163.155
331.795
129.173
259
95
249.5
51
12
-3.62E-01
0
320.042
195.901
333.064
159.778
252
126.5
233
79
13
-7.13E-01
0
1533.78
179.705
2354.72
166.809
1459
98
2307
85
14
1.36E+00
0
229.661
183.158
131.155
155.087
144
101
63
73
15
-5.44E-01
0
251.471
217.185
180.701
114.732
187.5
154.5
110.5
39.5
16
1.14E-01
0
222.754
166.871
168.123
128.378
151
111
91
60
17
3.55E-01
0
604.697
208.368
435.949
118.569
527.5
133
296.5
53.5
18
-1.19E-01
0
1648.57
247.708
1666.18
161.175
1555
178
1489
81.5
19
3.21E-01
0
253.569
200.91
187.004
155.437
170
130
102.5
84
20
-3.56E-01
0
265.177
198.369
208.142
135.256
187
148
119
61
Total number of rows: 16928 Table truncated, full table size 1089 Kbytes .Supplementary data files not provided