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Status
Public on May 21, 2004
Title
A chip: time 5 day Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-5.45E-01
0
587.566
268.456
742.663
413.434
485
155
672
308
2
3.21E-01
0
342.479
292.077
721.528
725.22
216
187.5
607
617.5
3
-4.96E-01
0
2076.32
268.061
1922.68
328.491
1714
170.5
1634
219.5
4
-1.66E+00
0
305.463
314.298
298.675
214.921
198
224
181.5
105.5
5
-6.48E-01
0
5802.58
373.835
5130.99
288.459
5310.5
259.5
4843
177.5
6
-1.63E-01
0
714.492
309.281
529.373
210.18
591
193.5
372
121
7
-3.98E-01
0
386.729
283.18
311.75
186.953
259
177.5
203
105
8
-1.17E-01
0
268.617
246.99
213.335
183.165
158
150
108
102
9
-5.94E-01
0
1831.38
277.948
1728.33
206.083
1417
158.5
1350
125.5
10
-1.08E-01
0
320.059
295.76
225.719
215.02
192.5
196.5
134
112.5
11
-3.30E-01
0
428.734
254.39
376.12
203.866
282
131
236
99
12
-5.15E-01
0
510.894
275.994
470.887
229.609
421
159.5
347
113
13
-7.63E-01
0
2501.44
324.988
2486.76
213.894
2398
208
2407
114
14
1.19E-01
0
269.635
266.982
188.098
168.345
157
162
106
92.5
15
-1.43E-01
0
300.293
273.279
236.948
189.385
193
142
126
100
16
2.83E-02
0
343.587
256.313
242.118
200.521
223
143
134
90.5
17
-1.38E-01
0
652.577
259.982
497.554
182.04
520
141
363
102
18
-5.83E-01
0
1716.35
254.301
1628.69
231.459
1563
143
1421
124.5
19
1.01E-01
0
416.523
247.617
298.911
195.96
262
143
152
101
20
-5.18E-02
0
350.008
251.965
252.932
169.643
235.5
135.5
141.5
84.5
Total number of rows: 16928 Table truncated, full table size 1098 Kbytes .Supplementary data files not provided