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Sample GSM22753 Query DataSets for GSM22753
Status Public on May 21, 2004
Title A chip: time 5 day Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -5.45E-01 0 587.566 268.456 742.663 413.434 485 155 672 308
2 3.21E-01 0 342.479 292.077 721.528 725.22 216 187.5 607 617.5
3 -4.96E-01 0 2076.32 268.061 1922.68 328.491 1714 170.5 1634 219.5
4 -1.66E+00 0 305.463 314.298 298.675 214.921 198 224 181.5 105.5
5 -6.48E-01 0 5802.58 373.835 5130.99 288.459 5310.5 259.5 4843 177.5
6 -1.63E-01 0 714.492 309.281 529.373 210.18 591 193.5 372 121
7 -3.98E-01 0 386.729 283.18 311.75 186.953 259 177.5 203 105
8 -1.17E-01 0 268.617 246.99 213.335 183.165 158 150 108 102
9 -5.94E-01 0 1831.38 277.948 1728.33 206.083 1417 158.5 1350 125.5
10 -1.08E-01 0 320.059 295.76 225.719 215.02 192.5 196.5 134 112.5
11 -3.30E-01 0 428.734 254.39 376.12 203.866 282 131 236 99
12 -5.15E-01 0 510.894 275.994 470.887 229.609 421 159.5 347 113
13 -7.63E-01 0 2501.44 324.988 2486.76 213.894 2398 208 2407 114
14 1.19E-01 0 269.635 266.982 188.098 168.345 157 162 106 92.5
15 -1.43E-01 0 300.293 273.279 236.948 189.385 193 142 126 100
16 2.83E-02 0 343.587 256.313 242.118 200.521 223 143 134 90.5
17 -1.38E-01 0 652.577 259.982 497.554 182.04 520 141 363 102
18 -5.83E-01 0 1716.35 254.301 1628.69 231.459 1563 143 1421 124.5
19 1.01E-01 0 416.523 247.617 298.911 195.96 262 143 152 101
20 -5.18E-02 0 350.008 251.965 252.932 169.643 235.5 135.5 141.5 84.5

Total number of rows: 16928

Table truncated, full table size 1098 Kbytes.




Supplementary data files not provided

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