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Status
Public on May 21, 2004
Title
A chip: time 5 day Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-7.00E-01
0
324.97
264.759
374.639
234.346
205
149
262
135
2
-9.14E-01
0
301.222
262.765
399.736
413.221
182
175
268
213.5
3
-5.08E-01
0
1645.75
299.434
1839.24
298.219
1464
169
1606
177
4
1.90E-02
0
314.07
239.244
262.724
181.036
209
126
143
109
5
-4.23E-01
0
4028.23
311.836
3644.04
259.746
3769.5
189
3248.5
157
6
-3.30E-02
0
442.751
250.148
365.097
190.341
303
145
218
102
7
1.38E-01
0
334.767
233.105
265.656
213.963
220.5
135
152.5
113.5
8
4.34E-01
0
266.976
234.349
197.741
216.562
155
129
105
107
9
-3.61E-01
0
1111.79
261.438
1085.15
235.863
922
177
872.5
122
10
-3.59E-02
0
288.12
277.826
226.695
210.55
181
167
124.5
120
11
-2.49E-01
0
423.856
270.066
388.393
205.265
294
153.5
268
114
12
-2.69E-01
0
369.483
297.716
307.526
224.395
231
193
185.5
132.5
13
-7.60E-01
0
899.124
298.943
1103.85
230.8
795
169.5
854
134.5
14
-1.45E-01
0
252.765
284.934
204.401
213.305
138
179
109
121
15
-6.13E-01
0
264.378
289.554
222.985
200.764
150
178
111
104
16
1.12E-01
0
311.153
252.141
225.264
196.127
179
138.5
121
93.5
17
4.17E-01
0
547.798
287.399
364.712
201.907
443
168
243
114
18
-2.74E-01
0
1232.91
303.142
1121.2
196.389
1119.5
192.5
989.5
105.5
19
-3.79E-01
0
317.048
308.357
211.041
177.057
189.5
205.5
113.5
90
20
-1.53E-01
0
272.167
227.1
236.572
181.154
163
126
128
104
Total number of rows: 16928 Table truncated, full table size 1098 Kbytes .Supplementary data files not provided