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Sample GSM22752 Query DataSets for GSM22752
Status Public on May 21, 2004
Title A chip: time 5 day Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -7.00E-01 0 324.97 264.759 374.639 234.346 205 149 262 135
2 -9.14E-01 0 301.222 262.765 399.736 413.221 182 175 268 213.5
3 -5.08E-01 0 1645.75 299.434 1839.24 298.219 1464 169 1606 177
4 1.90E-02 0 314.07 239.244 262.724 181.036 209 126 143 109
5 -4.23E-01 0 4028.23 311.836 3644.04 259.746 3769.5 189 3248.5 157
6 -3.30E-02 0 442.751 250.148 365.097 190.341 303 145 218 102
7 1.38E-01 0 334.767 233.105 265.656 213.963 220.5 135 152.5 113.5
8 4.34E-01 0 266.976 234.349 197.741 216.562 155 129 105 107
9 -3.61E-01 0 1111.79 261.438 1085.15 235.863 922 177 872.5 122
10 -3.59E-02 0 288.12 277.826 226.695 210.55 181 167 124.5 120
11 -2.49E-01 0 423.856 270.066 388.393 205.265 294 153.5 268 114
12 -2.69E-01 0 369.483 297.716 307.526 224.395 231 193 185.5 132.5
13 -7.60E-01 0 899.124 298.943 1103.85 230.8 795 169.5 854 134.5
14 -1.45E-01 0 252.765 284.934 204.401 213.305 138 179 109 121
15 -6.13E-01 0 264.378 289.554 222.985 200.764 150 178 111 104
16 1.12E-01 0 311.153 252.141 225.264 196.127 179 138.5 121 93.5
17 4.17E-01 0 547.798 287.399 364.712 201.907 443 168 243 114
18 -2.74E-01 0 1232.91 303.142 1121.2 196.389 1119.5 192.5 989.5 105.5
19 -3.79E-01 0 317.048 308.357 211.041 177.057 189.5 205.5 113.5 90
20 -1.53E-01 0 272.167 227.1 236.572 181.154 163 126 128 104

Total number of rows: 16928

Table truncated, full table size 1098 Kbytes.




Supplementary data files not provided

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