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Sample GSM22750 Query DataSets for GSM22750
Status Public on May 21, 2004
Title A chip: time 5 day Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 5 day Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 3.12E-01 0 102.925 43.1844 106.096 83.5 33 7 28 14
2 -2.66E-01 0 72.9505 74.6099 96.9114 78.8865 13.5 11 25 20.5
3 1.86E+00 0 486.758 74.6615 307.769 86.6739 292 11 189 19.5
4 -1.33E-01 0 85.4411 82.3993 98.5982 74.5597 14 13.5 29.5 14.5
5 3.46E-01 0 766.77 105.595 1461.77 97.7929 639 29 1283 29
6 -1.14E-01 0 86.9982 62.6211 96.9854 59.5714 14 13 17 11
7 -6.57E-02 0 107.031 59.3416 145.741 55.8416 27 9.5 49 9.5
8 4.68E-01 0 74.8938 52.9706 53.9384 52.3922 12.5 7 8 7
9 -2.29E-01 0 352.007 71.428 967.29 53.2669 265 13 818 8
10 2.05E-01 0 81.0017 52.1209 76.1438 59.5065 11 7 11 9.5
11 3.07E-01 0 172.842 46.1988 248.601 57.354 72 6 164 8
12 3.16E-01 0 105.7 78.0738 91.9205 46.8745 23 13 17 7
13 -9.03E-02 0 96.5846 56.1403 124.606 52.4065 20 8 18 7
14 1.53E-02 0 64.7718 59.9482 59.6246 61.6555 10 9 8 8
15 -5.03E-01 0 56.4681 60.4406 71.0583 51.3168 9 8.5 10 7
16 -5.28E-02 0 85.2226 53.1591 97.5171 66.0871 18 7 20 9
17 7.05E-02 0 140.687 62.6084 214.211 57.2039 49 8 77 8
18 -3.36E-01 0 201.654 50.0106 490.923 69.1943 108 9 357 11
19 -6.42E-01 0 54.0765 57.4434 73.7978 53.3269 8 9 14 8
20 -2.69E-01 0 72.1682 49.9255 85.9745 63.1489 11 9.5 16 8

Total number of rows: 16928

Table truncated, full table size 1030 Kbytes.




Supplementary data files not provided

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