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Status
Public on May 21, 2004
Title
A chip: time 5 day Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 5 day Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
3.12E-01
0
102.925
43.1844
106.096
83.5
33
7
28
14
2
-2.66E-01
0
72.9505
74.6099
96.9114
78.8865
13.5
11
25
20.5
3
1.86E+00
0
486.758
74.6615
307.769
86.6739
292
11
189
19.5
4
-1.33E-01
0
85.4411
82.3993
98.5982
74.5597
14
13.5
29.5
14.5
5
3.46E-01
0
766.77
105.595
1461.77
97.7929
639
29
1283
29
6
-1.14E-01
0
86.9982
62.6211
96.9854
59.5714
14
13
17
11
7
-6.57E-02
0
107.031
59.3416
145.741
55.8416
27
9.5
49
9.5
8
4.68E-01
0
74.8938
52.9706
53.9384
52.3922
12.5
7
8
7
9
-2.29E-01
0
352.007
71.428
967.29
53.2669
265
13
818
8
10
2.05E-01
0
81.0017
52.1209
76.1438
59.5065
11
7
11
9.5
11
3.07E-01
0
172.842
46.1988
248.601
57.354
72
6
164
8
12
3.16E-01
0
105.7
78.0738
91.9205
46.8745
23
13
17
7
13
-9.03E-02
0
96.5846
56.1403
124.606
52.4065
20
8
18
7
14
1.53E-02
0
64.7718
59.9482
59.6246
61.6555
10
9
8
8
15
-5.03E-01
0
56.4681
60.4406
71.0583
51.3168
9
8.5
10
7
16
-5.28E-02
0
85.2226
53.1591
97.5171
66.0871
18
7
20
9
17
7.05E-02
0
140.687
62.6084
214.211
57.2039
49
8
77
8
18
-3.36E-01
0
201.654
50.0106
490.923
69.1943
108
9
357
11
19
-6.42E-01
0
54.0765
57.4434
73.7978
53.3269
8
9
14
8
20
-2.69E-01
0
72.1682
49.9255
85.9745
63.1489
11
9.5
16
8
Total number of rows: 16928 Table truncated, full table size 1030 Kbytes .Supplementary data files not provided