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Sample GSM22749 Query DataSets for GSM22749
Status Public on May 21, 2004
Title A chip: time 3 day Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -2.49E-02 0 1305.21 396.365 1336.95 431.115 1237 352 1111 352
2 -4.65E-01 0 1036.78 661.482 1303.84 813.239 957 571 1181.5 652
3 -1.04E-01 0 17107.1 543.601 13486.1 588.933 18527 474 13506 507
4 -2.09E-01 0 514.326 349.381 558.674 329.301 440 268 478 284.5
5 -1.20E+00 0 8417.82 438.192 13720 466.453 8091 406.5 12841 416.5
6 -7.71E-01 0 933.485 289.998 1540.27 323.618 799 211 1299 267
7 -4.58E-01 0 481.499 278.292 590.148 290.325 420 204 471 221
8 -1.49E+00 0 325.947 325.229 354.175 276.956 249 271 287 212.5
9 -1.49E+00 0 3283.13 287.223 6857.19 363.535 2587 223 5005 312
10 1.12E-01 0 384.243 250.835 432.785 304.292 318 166 341 248.5
11 -5.68E-01 0 757.259 298.672 1015.03 295.404 618.5 235 785.5 219.5
12 7.15E-01 0 2061.9 277.848 1278.69 278.735 1851 199.5 1069 219
13 -9.89E-01 0 5572.87 299.821 7897.98 341.972 5636 242 7662 275
14 NULL 0 264.128 269.263 216.807 251.449 178 184 145 190
15 -9.08E-02 0 386.897 255.137 380.003 254.127 303.5 186.5 325.5 185
16 -2.78E-01 0 372.454 249.984 372.733 240.606 306 188.5 298 169
17 -6.58E-01 0 1317.56 332.532 1848.38 308.086 1201 273 1633 239.5
18 -1.21E+00 0 2884.48 317.939 5055.58 331.914 2755 248.5 4743 272
19 -1.50E-01 0 540.497 314 529.825 283.331 467.5 237.5 449.5 206.5
20 -2.25E-02 0 587.507 272.989 580.746 272.916 489 205 440 205

Total number of rows: 16928

Table truncated, full table size 1107 Kbytes.




Supplementary data files not provided

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