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Status
Public on May 21, 2004
Title
A chip: time 3 day Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-2.49E-02
0
1305.21
396.365
1336.95
431.115
1237
352
1111
352
2
-4.65E-01
0
1036.78
661.482
1303.84
813.239
957
571
1181.5
652
3
-1.04E-01
0
17107.1
543.601
13486.1
588.933
18527
474
13506
507
4
-2.09E-01
0
514.326
349.381
558.674
329.301
440
268
478
284.5
5
-1.20E+00
0
8417.82
438.192
13720
466.453
8091
406.5
12841
416.5
6
-7.71E-01
0
933.485
289.998
1540.27
323.618
799
211
1299
267
7
-4.58E-01
0
481.499
278.292
590.148
290.325
420
204
471
221
8
-1.49E+00
0
325.947
325.229
354.175
276.956
249
271
287
212.5
9
-1.49E+00
0
3283.13
287.223
6857.19
363.535
2587
223
5005
312
10
1.12E-01
0
384.243
250.835
432.785
304.292
318
166
341
248.5
11
-5.68E-01
0
757.259
298.672
1015.03
295.404
618.5
235
785.5
219.5
12
7.15E-01
0
2061.9
277.848
1278.69
278.735
1851
199.5
1069
219
13
-9.89E-01
0
5572.87
299.821
7897.98
341.972
5636
242
7662
275
14
NULL
0
264.128
269.263
216.807
251.449
178
184
145
190
15
-9.08E-02
0
386.897
255.137
380.003
254.127
303.5
186.5
325.5
185
16
-2.78E-01
0
372.454
249.984
372.733
240.606
306
188.5
298
169
17
-6.58E-01
0
1317.56
332.532
1848.38
308.086
1201
273
1633
239.5
18
-1.21E+00
0
2884.48
317.939
5055.58
331.914
2755
248.5
4743
272
19
-1.50E-01
0
540.497
314
529.825
283.331
467.5
237.5
449.5
206.5
20
-2.25E-02
0
587.507
272.989
580.746
272.916
489
205
440
205
Total number of rows: 16928 Table truncated, full table size 1107 Kbytes .Supplementary data files not provided