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Status
Public on May 21, 2004
Title
A chip: time 3 day Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-0.166152545
0
574.301
403.238
380.53
313.494
471
279
232
166
2
-0.753212258
0
603.394
738.029
476.812
516.859
481.5
565
357
383
3
0.507358999
0
2777.28
455.96
1115.26
400.355
2179
335
885
292
4
-1.077896786
0
434.846
357.639
356.32
244.024
344
292
220
140
5
-1.203135748
0
5411.94
394.332
4437.89
314.742
5119
277
4012
205
6
-0.410125668
0
690.2
317.378
520.488
231.369
591
193
362
113
7
-0.705441599
0
502.671
332.876
387.343
225.324
380
234
254
109
8
-0.080305747
0
303.197
313.988
236.033
231.096
177.5
200.5
134
130
9
-1.474527923
0
2331.14
351.813
2559.28
239.683
2079
241.5
2258
149
10
-0.11356547
0
391.153
318.717
287.336
223.756
269
185.5
182
128
11
-0.390288993
0
653.619
361.048
496.696
237.102
530
220
342
144
12
0.899541146
0
1253.49
281.335
470.574
219.423
1034
172
334
123.5
13
-0.704221739
0
4115.31
324.013
2605.99
245.516
4097
202
2471
145
14
0.004033726
0
303.452
289.313
234.04
215.796
198
170
127
120.5
15
0.447239107
0
358.115
230.703
250.62
220.928
250
138
135
132
16
-0.375221505
0
394.293
290.301
320.776
249.558
266
182
198
130
17
-0.257634536
0
1076.25
326.979
728.981
222.878
918.5
202
537.5
122.5
18
-0.971998419
0
2259.85
326.301
1898.96
266.29
2098
209
1758
150
19
0.077038391
0
402.22
314.105
263.616
227.341
283
195.5
143
121.5
20
0.135460546
0
581.011
327.4
362.401
267.895
465
218
219
152
Total number of rows: 16928 Table truncated, full table size 1151 Kbytes .Supplementary data files not provided