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Sample GSM22744 Query DataSets for GSM22744
Status Public on May 21, 2004
Title A chip: time 3 day Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 day Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -6.39E-01 0 67.4769 73.9063 129.816 123.5 11 12 47 34
2 1.11E-02 0 89.4229 76.8409 134.753 128.174 15 13.5 53 49.5
3 6.07E-01 0 298.51 63.1643 320.614 126.406 177 9 211 43
4 -6.84E-01 0 58.6685 59.7333 114.787 97.0275 8 9 27 25
5 -7.22E-01 0 355.741 90.8932 821.993 95.5016 267 15 655 24
6 -2.11E-01 0 72.1652 57.6241 96.8649 82.6135 11 8 24.5 14
7 -5.67E-01 0 72.3018 58.4161 114.122 63.7587 10 9 33.5 8.5
8 -3.70E-01 0 58.938 44.788 85.8696 80.9682 8 8 15 13
9 -2.78E-01 0 340.171 55.594 602.51 80.802 262 9 479 21
10 -2.81E-01 0 61.5781 50.6844 86.452 75.5496 10 6.5 17 11
11 1.98E-02 0 134.459 51.786 192.407 85.2096 44 6 90 16
12 1.76E-01 0 111.404 45.9579 128.96 87.6375 24 7 27 16
13 -1.60E-01 0 88.6769 61.1832 115.615 84.6708 18 9 22 13
14 -1.12E-01 0 63.9636 56.2574 77.1894 77.3663 10 9 13 11.5
15 -1.16E-02 0 62.3682 57.8871 72.5822 63.1452 8 7 10.5 11.5
16 -4.27E-01 0 64.2395 59.2075 105.106 97.6509 9 9 28 21.5
17 -1.31E-01 0 138.749 62.2381 210.497 70.8912 48 9 107 11
18 1.68E-03 0 259.838 63.3356 374.794 70.5134 162 10.5 267 13
19 -2.02E-01 0 56.979 62.5804 66.8258 51.3042 8 8 10 7
20 2.00E-02 0 77.2497 51.7112 90.4732 77.1551 10 8 23 14

Total number of rows: 16928

Table truncated, full table size 1006 Kbytes.




Supplementary data files not provided

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