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Status
Public on May 21, 2004
Title
A chip: time 3 day Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 day Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-6.39E-01
0
67.4769
73.9063
129.816
123.5
11
12
47
34
2
1.11E-02
0
89.4229
76.8409
134.753
128.174
15
13.5
53
49.5
3
6.07E-01
0
298.51
63.1643
320.614
126.406
177
9
211
43
4
-6.84E-01
0
58.6685
59.7333
114.787
97.0275
8
9
27
25
5
-7.22E-01
0
355.741
90.8932
821.993
95.5016
267
15
655
24
6
-2.11E-01
0
72.1652
57.6241
96.8649
82.6135
11
8
24.5
14
7
-5.67E-01
0
72.3018
58.4161
114.122
63.7587
10
9
33.5
8.5
8
-3.70E-01
0
58.938
44.788
85.8696
80.9682
8
8
15
13
9
-2.78E-01
0
340.171
55.594
602.51
80.802
262
9
479
21
10
-2.81E-01
0
61.5781
50.6844
86.452
75.5496
10
6.5
17
11
11
1.98E-02
0
134.459
51.786
192.407
85.2096
44
6
90
16
12
1.76E-01
0
111.404
45.9579
128.96
87.6375
24
7
27
16
13
-1.60E-01
0
88.6769
61.1832
115.615
84.6708
18
9
22
13
14
-1.12E-01
0
63.9636
56.2574
77.1894
77.3663
10
9
13
11.5
15
-1.16E-02
0
62.3682
57.8871
72.5822
63.1452
8
7
10.5
11.5
16
-4.27E-01
0
64.2395
59.2075
105.106
97.6509
9
9
28
21.5
17
-1.31E-01
0
138.749
62.2381
210.497
70.8912
48
9
107
11
18
1.68E-03
0
259.838
63.3356
374.794
70.5134
162
10.5
267
13
19
-2.02E-01
0
56.979
62.5804
66.8258
51.3042
8
8
10
7
20
2.00E-02
0
77.2497
51.7112
90.4732
77.1551
10
8
23
14
Total number of rows: 16928 Table truncated, full table size 1006 Kbytes .Supplementary data files not provided