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Sample GSM22742 Query DataSets for GSM22742
Status Public on May 21, 2004
Title A chip: time 24 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -0.218988468 0 381.685 244.805 740.526 549.943 292 168.5 665 495
2 -3.373754537 0 450.193 406.067 2000.39 1617.75 367 371 1864 1342
3 0.313398536 0 4520.08 285.682 4067.36 876.11 4458 203 3804 786
4 0.298539709 0 279.993 170.17 435.346 356.699 204 101 368 294
5 -0.659082134 0 6186.2 240.955 7967.66 342.795 6028 182 7390 272
6 -1.047406301 0 621.177 216.17 1171.55 253.948 544 149.5 901 183
7 -0.106119835 0 317.66 162.258 427.615 217.292 242 65 345 144
8 -0.318806649 0 184.404 204.995 203.543 248.325 98 125 124 132
9 -0.587669357 0 2432.34 206.217 3396.56 214.848 2014 134 2454 150
10 0.049941332 0 228.176 164.62 275.682 200.926 132.5 72.5 204 130.5
11 -0.277760086 0 524.809 177.952 730.821 241.638 441 81 610 171.5
12 0.867500938 0 1057.41 166.444 720.077 183.178 647 106 515 112
13 -0.679284783 0 2982.72 212.83 4250.79 235.245 2584 135.5 3524 150.5
14 0.275500671 0 186.856 177.636 197.67 189.492 101 85 131 114.5
15 0.116535525 0 226.58 180.277 241.17 208.242 153.5 109.5 173.5 134.5
16 -0.302509815 0 206.284 152.105 248.194 179.142 116.5 75 162.5 94
17 0.217465204 0 719.368 217.158 640.636 201.231 533 149 503 110
18 -0.078843666 0 1525.89 209.534 1706.85 190.011 1349 114 1486 117.5
19 -0.706800288 0 195.789 212.253 200.058 191.842 113 134 127 108
20 0.18516746 0 340.111 180.979 342.778 207.367 253 101 247 130

Total number of rows: 16928

Table truncated, full table size 1142 Kbytes.




Supplementary data files not provided

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