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Status
Public on May 21, 2004
Title
A chip: time 24 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-0.218988468
0
381.685
244.805
740.526
549.943
292
168.5
665
495
2
-3.373754537
0
450.193
406.067
2000.39
1617.75
367
371
1864
1342
3
0.313398536
0
4520.08
285.682
4067.36
876.11
4458
203
3804
786
4
0.298539709
0
279.993
170.17
435.346
356.699
204
101
368
294
5
-0.659082134
0
6186.2
240.955
7967.66
342.795
6028
182
7390
272
6
-1.047406301
0
621.177
216.17
1171.55
253.948
544
149.5
901
183
7
-0.106119835
0
317.66
162.258
427.615
217.292
242
65
345
144
8
-0.318806649
0
184.404
204.995
203.543
248.325
98
125
124
132
9
-0.587669357
0
2432.34
206.217
3396.56
214.848
2014
134
2454
150
10
0.049941332
0
228.176
164.62
275.682
200.926
132.5
72.5
204
130.5
11
-0.277760086
0
524.809
177.952
730.821
241.638
441
81
610
171.5
12
0.867500938
0
1057.41
166.444
720.077
183.178
647
106
515
112
13
-0.679284783
0
2982.72
212.83
4250.79
235.245
2584
135.5
3524
150.5
14
0.275500671
0
186.856
177.636
197.67
189.492
101
85
131
114.5
15
0.116535525
0
226.58
180.277
241.17
208.242
153.5
109.5
173.5
134.5
16
-0.302509815
0
206.284
152.105
248.194
179.142
116.5
75
162.5
94
17
0.217465204
0
719.368
217.158
640.636
201.231
533
149
503
110
18
-0.078843666
0
1525.89
209.534
1706.85
190.011
1349
114
1486
117.5
19
-0.706800288
0
195.789
212.253
200.058
191.842
113
134
127
108
20
0.18516746
0
340.111
180.979
342.778
207.367
253
101
247
130
Total number of rows: 16928 Table truncated, full table size 1142 Kbytes .Supplementary data files not provided