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Sample GSM22739 Query DataSets for GSM22739
Status Public on May 21, 2004
Title A chip: time 24 hour Replicate 1
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 24 hour Replicate 1
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 0 903.459 321.033 849.015 331.193 825 243.5 713 297.5
2 -1.34E-01 0 966.61 700.318 950.361 831.324 885 462 886.5 541
3 3.42E-02 0 7175.26 373.656 5028.98 366.637 7406 345 4929 277.5
4 -5.32E-01 0 570.073 264.232 510.122 177.899 460 188 403 116
5 -2.92E-01 0 16644.4 480.126 11664.2 422.6 16328 400.5 11810 365
6 -2.89E-01 0 1130.26 259.671 977.299 214.263 1009 217 791 147
7 -2.04E-01 0 920.448 241.172 729.118 153.098 808 188 576 91.5
8 -4.98E-01 0 187.577 210.464 115.55 131.061 115 141 54 42
9 -2.32E-01 0 6740.2 265.52 5476.48 235.88 5589 210 4697 155
10 5.44E-03 0 580.862 195.275 422.98 151.686 322 122 211 81.5
11 -2.97E-01 0 2177.07 225.845 1860.61 158.707 2063 150 1685 89
12 -1.52E-02 0 795.053 178.965 596.069 129.237 451 100 289 59
13 -1.58E-02 0 6769.38 238.893 4836.03 151.865 5527 183 3733 105
14 3.77E-01 0 202.889 170.864 135.443 138.879 132 122.5 76 69.5
15 8.62E-01 0 225.205 216.809 181.012 207.489 170 136 111 125.5
16 -2.14E-01 0 319.633 251.955 227.878 165.173 242 182 141 95
17 2.00E-01 0 2256.05 213.277 1430.18 131.928 1956 133.5 1197 57.5
18 -3.90E-01 0 4040.39 234.247 3667.65 160.584 3953 168 3515 97
19 8.22E-01 0 215.614 199.459 127.492 142.459 149 159 55 83
20 1.35E-01 0 606.275 222.957 379.107 145.612 486 178 313 83

Total number of rows: 16928

Table truncated, full table size 1101 Kbytes.




Supplementary data files not provided

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