GEO help: Mouse over screen elements for information.
Status
Public on May 21, 2004
Title
A chip: time 24 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 24 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
0
903.459
321.033
849.015
331.193
825
243.5
713
297.5
2
-1.34E-01
0
966.61
700.318
950.361
831.324
885
462
886.5
541
3
3.42E-02
0
7175.26
373.656
5028.98
366.637
7406
345
4929
277.5
4
-5.32E-01
0
570.073
264.232
510.122
177.899
460
188
403
116
5
-2.92E-01
0
16644.4
480.126
11664.2
422.6
16328
400.5
11810
365
6
-2.89E-01
0
1130.26
259.671
977.299
214.263
1009
217
791
147
7
-2.04E-01
0
920.448
241.172
729.118
153.098
808
188
576
91.5
8
-4.98E-01
0
187.577
210.464
115.55
131.061
115
141
54
42
9
-2.32E-01
0
6740.2
265.52
5476.48
235.88
5589
210
4697
155
10
5.44E-03
0
580.862
195.275
422.98
151.686
322
122
211
81.5
11
-2.97E-01
0
2177.07
225.845
1860.61
158.707
2063
150
1685
89
12
-1.52E-02
0
795.053
178.965
596.069
129.237
451
100
289
59
13
-1.58E-02
0
6769.38
238.893
4836.03
151.865
5527
183
3733
105
14
3.77E-01
0
202.889
170.864
135.443
138.879
132
122.5
76
69.5
15
8.62E-01
0
225.205
216.809
181.012
207.489
170
136
111
125.5
16
-2.14E-01
0
319.633
251.955
227.878
165.173
242
182
141
95
17
2.00E-01
0
2256.05
213.277
1430.18
131.928
1956
133.5
1197
57.5
18
-3.90E-01
0
4040.39
234.247
3667.65
160.584
3953
168
3515
97
19
8.22E-01
0
215.614
199.459
127.492
142.459
149
159
55
83
20
1.35E-01
0
606.275
222.957
379.107
145.612
486
178
313
83
Total number of rows: 16928 Table truncated, full table size 1101 Kbytes .Supplementary data files not provided