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Status
Public on May 21, 2004
Title
A chip: time 8 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.41E-02
0
247.836
166.377
250.57
161.576
141
73
165
76
2
NULL
0
258.202
270.929
463.459
517.548
182
181
404
422.5
3
4.18E-01
0
2334.7
213.349
2081.86
490.782
2281
117.5
1910
441.5
4
6.44E-01
0
194.772
138.07
356.887
313.668
112
57.5
293
273.5
5
8.72E-02
0
3317.55
173.668
3037.01
230.574
2978
91
2721
162.5
6
-7.18E-02
0
348.026
127.409
430.952
214.702
251
59.5
337
107.5
7
-2.63E-01
0
216.056
112.033
305.852
158.541
115
30
226
77
8
NULL
0
126.67
132.2
170.434
136.836
41
41
88
76
9
-8.60E-01
0
1223.36
186.072
2060.17
194.606
939
107.5
1446
96
10
4.73E-01
0
155.8
128.311
163.955
188.503
73
50
77
89
11
-5.96E-01
0
284.267
128.705
412.651
125.248
198
47.5
320
38
12
6.61E-01
0
749.721
137.996
578.281
145.247
535
43
421
70
13
-9.92E-01
0
889.262
116.112
1735.63
134.324
719
36
1382
64
14
-1.25E-02
0
143.34
142.741
124.981
117.211
48
57
45
40
15
3.06E-01
0
138.308
128.637
146.705
164.119
48.5
50.5
56
75.5
16
-8.44E-02
0
168.378
131.654
169.089
144.197
86
50.5
83
51.5
17
-1.06E-01
0
466.078
142.439
558.741
121.561
306
45.5
380
49.5
18
-4.06E-01
0
1081.93
112.476
1454.5
137.845
866
32
1129
62
19
8.18E-01
0
136.785
109.106
131.082
130.336
57
28
50
69
20
-2.28E-01
0
229.144
184.311
202.082
117.693
121
97
126
52
Total number of rows: 16928 Table truncated, full table size 1054 Kbytes .Supplementary data files not provided