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Sample GSM22737 Query DataSets for GSM22737
Status Public on May 21, 2004
Title A chip: time 8 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.41E-02 0 247.836 166.377 250.57 161.576 141 73 165 76
2 NULL 0 258.202 270.929 463.459 517.548 182 181 404 422.5
3 4.18E-01 0 2334.7 213.349 2081.86 490.782 2281 117.5 1910 441.5
4 6.44E-01 0 194.772 138.07 356.887 313.668 112 57.5 293 273.5
5 8.72E-02 0 3317.55 173.668 3037.01 230.574 2978 91 2721 162.5
6 -7.18E-02 0 348.026 127.409 430.952 214.702 251 59.5 337 107.5
7 -2.63E-01 0 216.056 112.033 305.852 158.541 115 30 226 77
8 NULL 0 126.67 132.2 170.434 136.836 41 41 88 76
9 -8.60E-01 0 1223.36 186.072 2060.17 194.606 939 107.5 1446 96
10 4.73E-01 0 155.8 128.311 163.955 188.503 73 50 77 89
11 -5.96E-01 0 284.267 128.705 412.651 125.248 198 47.5 320 38
12 6.61E-01 0 749.721 137.996 578.281 145.247 535 43 421 70
13 -9.92E-01 0 889.262 116.112 1735.63 134.324 719 36 1382 64
14 -1.25E-02 0 143.34 142.741 124.981 117.211 48 57 45 40
15 3.06E-01 0 138.308 128.637 146.705 164.119 48.5 50.5 56 75.5
16 -8.44E-02 0 168.378 131.654 169.089 144.197 86 50.5 83 51.5
17 -1.06E-01 0 466.078 142.439 558.741 121.561 306 45.5 380 49.5
18 -4.06E-01 0 1081.93 112.476 1454.5 137.845 866 32 1129 62
19 8.18E-01 0 136.785 109.106 131.082 130.336 57 28 50 69
20 -2.28E-01 0 229.144 184.311 202.082 117.693 121 97 126 52

Total number of rows: 16928

Table truncated, full table size 1054 Kbytes.




Supplementary data files not provided

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