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Sample GSM22735 Query DataSets for GSM22735
Status Public on May 21, 2004
Title A chip: time 8 hour Replicate 3
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 8 hour Replicate 3
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 2.59E-01 0 659.907 323.926 371.177 218.872 551 191.5 209 132
2 4.04E-01 0 876.248 435.977 460.758 317.887 736 294 336 196
3 4.64E-01 0 9987.73 352.424 3064.85 252.01 10576 215 2870 125
4 8.70E-02 0 462.288 284.889 283.159 190.022 322.5 169 159.5 109.5
5 3.92E-01 0 12883.1 392.767 4090.73 268.411 13190 291 3702 149
6 2.06E-01 0 1257.95 308.413 604.832 201.394 1109 188 412 108
7 -4.92E-01 0 531.237 247.376 430.554 216.275 355 143 261 115
8 -2.88E-01 0 269.462 282.795 223.688 221.803 152.5 148.5 110.5 103
9 -7.29E-01 0 3573.25 264.359 2435.81 244.649 2697 164 1725 139
10 -2.70E-01 0 321.734 243.184 249.601 182.38 190 143 148 100
11 -1.10E+00 0 616.244 287.059 623.344 227.111 478 164 448 114
12 8.97E-01 0 2717.38 263.451 807.1 202.988 1916 177 585 102
13 -1.49E+00 0 3199.06 272.139 3380.25 234.455 3143 150.5 3014 130.5
14 -2.40E-01 0 279.877 217.94 223.098 196.187 168.5 147 121 99
15 -2.69E-01 0 302.288 253.306 241.2 187.934 187 134.5 127 97.5
16 -6.47E-01 0 303.985 251.478 268.605 189.544 197 144 163 99.5
17 3.59E-01 0 1222.92 279.845 564.701 216.41 885 174 382 119
18 -2.48E-01 0 2692.67 265.695 1453.86 213.43 2199 162 1074 108
19 -1.12E-02 0 269.974 252.768 206.553 192.212 166 135 106 94.5
20 5.55E-01 0 646.33 274.575 323.31 204.095 513 154 180 114

Total number of rows: 16928

Table truncated, full table size 1096 Kbytes.




Supplementary data files not provided

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