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Status
Public on May 21, 2004
Title
A chip: time 8 hour Replicate 3
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 8 hour Replicate 3
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
2.59E-01
0
659.907
323.926
371.177
218.872
551
191.5
209
132
2
4.04E-01
0
876.248
435.977
460.758
317.887
736
294
336
196
3
4.64E-01
0
9987.73
352.424
3064.85
252.01
10576
215
2870
125
4
8.70E-02
0
462.288
284.889
283.159
190.022
322.5
169
159.5
109.5
5
3.92E-01
0
12883.1
392.767
4090.73
268.411
13190
291
3702
149
6
2.06E-01
0
1257.95
308.413
604.832
201.394
1109
188
412
108
7
-4.92E-01
0
531.237
247.376
430.554
216.275
355
143
261
115
8
-2.88E-01
0
269.462
282.795
223.688
221.803
152.5
148.5
110.5
103
9
-7.29E-01
0
3573.25
264.359
2435.81
244.649
2697
164
1725
139
10
-2.70E-01
0
321.734
243.184
249.601
182.38
190
143
148
100
11
-1.10E+00
0
616.244
287.059
623.344
227.111
478
164
448
114
12
8.97E-01
0
2717.38
263.451
807.1
202.988
1916
177
585
102
13
-1.49E+00
0
3199.06
272.139
3380.25
234.455
3143
150.5
3014
130.5
14
-2.40E-01
0
279.877
217.94
223.098
196.187
168.5
147
121
99
15
-2.69E-01
0
302.288
253.306
241.2
187.934
187
134.5
127
97.5
16
-6.47E-01
0
303.985
251.478
268.605
189.544
197
144
163
99.5
17
3.59E-01
0
1222.92
279.845
564.701
216.41
885
174
382
119
18
-2.48E-01
0
2692.67
265.695
1453.86
213.43
2199
162
1074
108
19
-1.12E-02
0
269.974
252.768
206.553
192.212
166
135
106
94.5
20
5.55E-01
0
646.33
274.575
323.31
204.095
513
154
180
114
Total number of rows: 16928 Table truncated, full table size 1096 Kbytes .Supplementary data files not provided