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Status
Public on May 21, 2004
Title
A chip: time 3 hour Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-2.80E-01
0
77.4849
61.7059
122.984
81.0727
12
9
43
20
2
-2.58E-01
0
85.1479
59.5991
139.065
103.694
16
7
46
23
3
-2.17E-01
0
255.618
56.1004
384.81
87.0743
151
8
308
25
4
-5.08E-01
0
75.502
68.0355
140.222
99.7234
12.5
9
67
23
5
2.53E-02
0
1258.75
100.671
1504.01
131.168
1072
28
1312
56
6
1.58E-01
0
141.087
65.3403
177.076
81.9757
47.5
8
87
17
7
-5.24E-01
0
88.765
69.9547
150.377
63.4302
19
9
59
8
8
3.31E-01
0
64.051
65.75
76.8506
88.5379
7
9
12
18.5
9
-2.29E-01
0
654.163
77.0164
972.244
116.461
482
13.5
754
33
10
-2.45E-01
0
91.9873
45.3669
143.959
82.7266
10
7
41
19
11
-9.14E-01
0
142.393
50.4539
334.371
90.2796
58.5
7
227
15
12
-6.23E-01
0
62.2397
52.7703
116.89
80.598
8
7
35
12.5
13
-2.60E-01
0
124.916
53.7621
199.368
72.869
39
7
74.5
12
14
NULL
0
57.1392
41.5576
112.352
66.8756
7
6
44
9
15
-2.55E-01
0
57.5291
46.7361
95.4506
82.4444
7
5
18
15
16
NULL
0
72.7409
55.85
115.606
79.7233
8
6.5
31
19
17
-5.69E-01
0
114.334
50.9369
217.652
59.0694
27
7
121
8
18
-4.66E-01
0
302.583
58.5347
526.959
68.1944
220
7
428.5
10
19
-1.54E-01
0
66.638
49.6723
97.6152
84.034
8
7
26
14
20
5.94E-01
0
70.0707
44.8357
86.5289
98.7286
10
5
18
29.5
Total number of rows: 16928 Table truncated, full table size 1026 Kbytes .Supplementary data files not provided