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Sample GSM22731 Query DataSets for GSM22731
Status Public on May 21, 2004
Title A chip: time 3 hour Replicate 5
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 -4.09E-01 0 76.3973 44.9907 152.942 121.696 14 7 74 38.5
2 6.13E-01 0 83.2277 67.5889 120.824 140.507 14 14 41.5 72
3 3.19E-01 0 239.989 56.6996 297.754 116.064 112 8 208 46
4 8.95E-02 0 88.1848 65.6423 137.6 138.87 20 11 71 47
5 7.93E-02 0 1425.81 89.9927 1666.37 136.04 1171 15 1368 62
6 1.11E+00 0 121.73 51.6882 148.095 145.141 29 8.5 73 83.5
7 -2.42E-01 0 89.1275 46.3696 173.373 115.87 15 7 89 52
8 -2.12E-01 0 65.5119 57.2399 102.978 99.2214 9 11 33 30
9 -2.15E-01 0 655.157 83.5654 1049.85 141.921 542 14 826 78
10 -1.71E-01 0 80.9454 64.8571 133.053 113.957 15 13 46 37.5
11 -6.92E-01 0 151.638 62.7516 344.281 95.3168 55 8 231 23
12 NULL 0 78.5082 58.2964 128.71 89.2134 9 9 44 19
13 NULL 0 110.601 50.5885 246.497 107.969 15 7.5 119 34.5
14 NULL 0 53.5994 51.8175 100.647 71.4867 7 9 25 10
15 -7.87E-02 0 70.9818 65.9433 97 71.0258 10 8 30 17
16 -6.92E-02 0 77.012 63.8357 122.024 102.661 14 8 40 32
17 -1.95E-01 0 199.053 63.5816 323.029 110.442 109 10 225 28
18 -1.50E-01 0 516.907 63.911 778.965 101.831 428 10 566 34.5
19 1.49E-01 0 65.4894 52.7774 94.1561 111.362 10 9 24 44
20 4.52E-01 0 78.976 70.8889 83.2462 97.4848 16 9.5 15 24.5

Total number of rows: 16928

Table truncated, full table size 1028 Kbytes.




Supplementary data files not provided

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