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Status
Public on May 21, 2004
Title
A chip: time 3 hour Replicate 5
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 5
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-4.09E-01
0
76.3973
44.9907
152.942
121.696
14
7
74
38.5
2
6.13E-01
0
83.2277
67.5889
120.824
140.507
14
14
41.5
72
3
3.19E-01
0
239.989
56.6996
297.754
116.064
112
8
208
46
4
8.95E-02
0
88.1848
65.6423
137.6
138.87
20
11
71
47
5
7.93E-02
0
1425.81
89.9927
1666.37
136.04
1171
15
1368
62
6
1.11E+00
0
121.73
51.6882
148.095
145.141
29
8.5
73
83.5
7
-2.42E-01
0
89.1275
46.3696
173.373
115.87
15
7
89
52
8
-2.12E-01
0
65.5119
57.2399
102.978
99.2214
9
11
33
30
9
-2.15E-01
0
655.157
83.5654
1049.85
141.921
542
14
826
78
10
-1.71E-01
0
80.9454
64.8571
133.053
113.957
15
13
46
37.5
11
-6.92E-01
0
151.638
62.7516
344.281
95.3168
55
8
231
23
12
NULL
0
78.5082
58.2964
128.71
89.2134
9
9
44
19
13
NULL
0
110.601
50.5885
246.497
107.969
15
7.5
119
34.5
14
NULL
0
53.5994
51.8175
100.647
71.4867
7
9
25
10
15
-7.87E-02
0
70.9818
65.9433
97
71.0258
10
8
30
17
16
-6.92E-02
0
77.012
63.8357
122.024
102.661
14
8
40
32
17
-1.95E-01
0
199.053
63.5816
323.029
110.442
109
10
225
28
18
-1.50E-01
0
516.907
63.911
778.965
101.831
428
10
566
34.5
19
1.49E-01
0
65.4894
52.7774
94.1561
111.362
10
9
24
44
20
4.52E-01
0
78.976
70.8889
83.2462
97.4848
16
9.5
15
24.5
Total number of rows: 16928 Table truncated, full table size 1028 Kbytes .Supplementary data files not provided