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Status
Public on May 21, 2004
Title
A chip: time 3 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
-1.37E-01
0
79.7938
59.6646
98.3869
70.8671
15
8
25.5
9
2
-4.64E-01
0
83.3596
64.9645
130.251
79.3652
20
10
43
14
3
4.18E-01
0
459.957
82.9444
497.457
97.4841
301
16.5
339
19
4
-6.26E-02
0
90.043
78.9752
116.166
85.3865
22.5
8.5
34.5
20
5
-5.86E-01
0
1602.17
122.208
3107.19
131.517
1447.5
33.5
2891.5
59.5
6
2.70E-01
0
121.262
61.8587
134.105
87.9591
35
9
40.5
17
7
-2.60E-01
0
92.8088
45.5777
133.532
66.9662
20
7
53
12
8
-3.89E-01
0
56.6207
64.0284
74.6355
59.4716
8
12.5
18.5
8.5
9
-2.69E-01
0
363.828
71.06
648.252
106.24
251.5
13.5
486.5
35.5
10
-1.12E-01
0
71.75
61.5987
88.7956
76.9547
12
8
17
12
11
-6.69E-01
0
96.5875
60.9659
192.455
50.572
25
9
93.5
8
12
6.98E-01
0
157.976
63.556
141.38
75.6791
49.5
8.5
42
20
13
-1.68E-01
0
190.288
60.097
307.122
66.5522
82.5
8
113.5
10.5
14
3.69E-01
0
88.9185
73.9776
80.5
64.0522
21.5
10
17
11.5
15
4.79E-01
0
85.731
51.771
75.837
74.743
18
6
17.5
12
16
4.18E-02
0
83.7706
57.32
99.1912
94.0222
15
7
20.5
15
17
1.20E-01
0
211.44
77.7077
275.656
76.6056
121.5
15
170.5
16
18
-1.19E-01
0
334.823
90.695
521.635
107.266
229
20
399
18.5
19
-1.09E-02
0
70.9544
71.3734
75.8759
59.7152
11
9
16
8
20
6.01E-01
0
91.9853
65.1894
78.2824
86.8598
22.5
10
14
18.5
Total number of rows: 16928 Table truncated, full table size 1045 Kbytes .Supplementary data files not provided