NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM22728 Query DataSets for GSM22728
Status Public on May 21, 2004
Title A chip: time 3 hour Replicate 2
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 3 hour Replicate 2
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 4.14E-01 0 161.026 93.1606 135.967 109.018 56 29 46 26
2 3.09E-02 0 109.397 85.75 105.528 75.839 24 19 38 13.5
3 1.11E+00 0 478.534 98.7685 314.459 97.547 317 27.5 207 19.5
4 -1.61E-01 0 139.871 126.672 134.278 90.1956 54 39 44 13
5 5.32E-02 0 2666.16 112.891 2819.05 139.418 2328 43 2355 73
6 5.01E-01 0 166.563 103.738 132.587 93.9709 70 27 38 21
7 -2.35E-01 0 122 77.4917 158.458 76.3208 39 16.5 71 19
8 1.71E-01 0 80.4785 68.64 91.2321 102.04 17 10 21 30
9 -1.46E-01 0 1508.31 100.598 2007.32 144.595 1060 30 1360 71
10 1.58E-01 0 121.383 68.177 137.578 107.121 41 10 53 31
11 -5.30E-03 0 222.61 70.5092 303.868 80.0996 137 12 204 16
12 -6.81E-02 0 113.962 95.8799 124.984 76.1696 27 16 36 16
13 4.76E-01 0 195.177 64.4441 181.925 80.2298 56 14 61 17
14 2.31E-01 0 97.4772 72.6473 91.9872 86.1345 22 11 21 17
15 1.93E-01 0 86.2252 47.1407 94.5818 83.9347 15 6 23 24
16 -4.76E-01 0 95.1164 80.3395 122.364 81.4074 23 21.5 41 16
17 -1.00E-01 0 249.61 68.3478 376.157 78.0217 141 8 236 13
18 -1.40E-01 0 458.716 96.9689 683.324 76.8354 382 27.5 498 16.5
19 3.11E-01 0 78.3524 85.4259 69.593 89.1963 16 15 14 21.5
20 -3.62E-01 0 84.4882 73.3741 108.359 71.3027 18 12 29 13

Total number of rows: 16928

Table truncated, full table size 1033 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap