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Status
Public on May 21, 2004
Title
A chip: time 3 hour Replicate 2
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 2
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
4.14E-01
0
161.026
93.1606
135.967
109.018
56
29
46
26
2
3.09E-02
0
109.397
85.75
105.528
75.839
24
19
38
13.5
3
1.11E+00
0
478.534
98.7685
314.459
97.547
317
27.5
207
19.5
4
-1.61E-01
0
139.871
126.672
134.278
90.1956
54
39
44
13
5
5.32E-02
0
2666.16
112.891
2819.05
139.418
2328
43
2355
73
6
5.01E-01
0
166.563
103.738
132.587
93.9709
70
27
38
21
7
-2.35E-01
0
122
77.4917
158.458
76.3208
39
16.5
71
19
8
1.71E-01
0
80.4785
68.64
91.2321
102.04
17
10
21
30
9
-1.46E-01
0
1508.31
100.598
2007.32
144.595
1060
30
1360
71
10
1.58E-01
0
121.383
68.177
137.578
107.121
41
10
53
31
11
-5.30E-03
0
222.61
70.5092
303.868
80.0996
137
12
204
16
12
-6.81E-02
0
113.962
95.8799
124.984
76.1696
27
16
36
16
13
4.76E-01
0
195.177
64.4441
181.925
80.2298
56
14
61
17
14
2.31E-01
0
97.4772
72.6473
91.9872
86.1345
22
11
21
17
15
1.93E-01
0
86.2252
47.1407
94.5818
83.9347
15
6
23
24
16
-4.76E-01
0
95.1164
80.3395
122.364
81.4074
23
21.5
41
16
17
-1.00E-01
0
249.61
68.3478
376.157
78.0217
141
8
236
13
18
-1.40E-01
0
458.716
96.9689
683.324
76.8354
382
27.5
498
16.5
19
3.11E-01
0
78.3524
85.4259
69.593
89.1963
16
15
14
21.5
20
-3.62E-01
0
84.4882
73.3741
108.359
71.3027
18
12
29
13
Total number of rows: 16928 Table truncated, full table size 1033 Kbytes .Supplementary data files not provided