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Status
Public on May 21, 2004
Title
A chip: time 3 hour Replicate 1
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 3 hour Replicate 1
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.65E-01
0
97.2956
65.7117
95.7774
78.6014
22
12
27
13
2
-8.73E-02
0
73.9504
59.1932
80.1674
79.8598
10
10
17
12
3
7.00E-01
0
224.206
79.3083
216.558
95.1053
130.5
17
143
25
4
-2.74E-01
0
84.4161
67.473
108.571
71.3716
18
11
35.5
12
5
-3.15E-01
0
739.172
109.937
1390.12
110.146
617
29
1042
37.5
6
2.51E-01
0
95.3096
68.124
90.9263
72.756
19
10
18
12.5
7
-3.49E-01
0
83.9209
57.7261
117.737
66.7357
15
8
32
11
8
3.39E-01
0
94.4244
78.4211
76.6395
70.2256
23
15
10
12
9
3.45E-01
0
566.385
91.8782
712.119
85.2941
404
18.5
431
17.5
10
-2.63E-02
0
78.9532
73.7184
90.6503
73.5345
17
11
25
20.5
11
-2.72E-01
0
121.184
56.1939
204.146
88.6224
28
10
92
25.5
12
NULL
0
77.2664
62.7833
128.214
64.3498
12
10
39
10
13
2.18E-01
0
118.664
65.5486
118.942
57
26
11
36
9
14
1.76E-02
0
74.722
73.5414
70.728
60.8232
10
13
9
10
15
-1.30E-01
0
73.9186
61.797
85.3576
73.1203
11
10
15.5
15
16
-2.39E-01
0
72.1547
67.3522
85.5612
69.8866
10
9
20.5
10
17
-6.80E-02
0
146.624
54.9439
218.071
89.2706
51
10
111
15
18
-2.45E-02
0
253.842
80.125
393.698
69.4964
148
17
282
8
19
-2.46E-02
0
76.3562
46.9853
81.0923
53.9228
14
7
15
9
20
NULL
0
63.7302
54.1567
95.1297
83.7788
9
7
27
13
Total number of rows: 16928 Table truncated, full table size 1032 Kbytes .Supplementary data files not provided