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Sample GSM22725 Query DataSets for GSM22725
Status Public on May 21, 2004
Title A chip: time 1 hour Replicate 4
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 1 hour Replicate 4
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 NULL 0 367.884 292.789 318.868 235.895 229 172.5 202.5 118
2 7.73E-02 0 397.894 319.191 321.539 353.618 285 208 187 189
3 8.31E-01 0 4327.34 324.346 1437.75 222.23 4368 219 1284 131
4 -8.18E-02 0 334.991 250.122 243.472 197.4 217 157 122 105
5 -6.52E-01 0 6377.61 329.845 4634.84 215.53 5922 211 4191 121
6 1.06E-01 0 719.534 276.393 493.472 202.246 590 161.5 337 106.5
7 -1.36E-01 0 405.34 215.179 312.31 162.96 291 114 185 85
8 3.18E-01 0 252.272 207.011 180.981 163.996 143 112 93 100
9 -7.35E-01 0 2450.6 235.362 2287.76 194.842 1987 133.5 1728 107.5
10 1.05E-01 0 248.984 196.407 193.512 181.516 134 105 105 99
11 -6.77E-01 0 432.716 199.733 479.74 181.737 311 106.5 316 102.5
12 9.92E-01 0 947.714 231.276 413.816 187.485 645 125 262 100.5
13 -9.63E-01 0 1658.26 207.233 1928.81 196.049 1457 119 1827 113
14 -5.63E-02 0 225.978 217.269 177.221 141.396 128 99 95 83.5
15 -1.09E-01 0 259.253 228.653 202.34 205.826 153 121 107 97
16 3.99E-01 0 284.628 197.209 203.115 197.289 169 108 107 107
17 3.84E-01 0 692.421 243.651 433.217 215.69 486 153 268 129
18 -3.49E-01 0 1620.46 232.506 1342.37 204.261 1319 127 953 121
19 7.32E-01 0 229.78 225.007 169.005 189.17 123 125 91 109.5
20 2.60E-01 0 456.06 231.915 278.835 163.718 324 131.5 146 86

Total number of rows: 16928

Table truncated, full table size 1086 Kbytes.




Supplementary data files not provided

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