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Status
Public on May 21, 2004
Title
A chip: time 1 hour Replicate 4
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 1 hour Replicate 4
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
NULL
0
367.884
292.789
318.868
235.895
229
172.5
202.5
118
2
7.73E-02
0
397.894
319.191
321.539
353.618
285
208
187
189
3
8.31E-01
0
4327.34
324.346
1437.75
222.23
4368
219
1284
131
4
-8.18E-02
0
334.991
250.122
243.472
197.4
217
157
122
105
5
-6.52E-01
0
6377.61
329.845
4634.84
215.53
5922
211
4191
121
6
1.06E-01
0
719.534
276.393
493.472
202.246
590
161.5
337
106.5
7
-1.36E-01
0
405.34
215.179
312.31
162.96
291
114
185
85
8
3.18E-01
0
252.272
207.011
180.981
163.996
143
112
93
100
9
-7.35E-01
0
2450.6
235.362
2287.76
194.842
1987
133.5
1728
107.5
10
1.05E-01
0
248.984
196.407
193.512
181.516
134
105
105
99
11
-6.77E-01
0
432.716
199.733
479.74
181.737
311
106.5
316
102.5
12
9.92E-01
0
947.714
231.276
413.816
187.485
645
125
262
100.5
13
-9.63E-01
0
1658.26
207.233
1928.81
196.049
1457
119
1827
113
14
-5.63E-02
0
225.978
217.269
177.221
141.396
128
99
95
83.5
15
-1.09E-01
0
259.253
228.653
202.34
205.826
153
121
107
97
16
3.99E-01
0
284.628
197.209
203.115
197.289
169
108
107
107
17
3.84E-01
0
692.421
243.651
433.217
215.69
486
153
268
129
18
-3.49E-01
0
1620.46
232.506
1342.37
204.261
1319
127
953
121
19
7.32E-01
0
229.78
225.007
169.005
189.17
123
125
91
109.5
20
2.60E-01
0
456.06
231.915
278.835
163.718
324
131.5
146
86
Total number of rows: 16928 Table truncated, full table size 1086 Kbytes .Supplementary data files not provided