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Status
Public on May 21, 2004
Title
A chip: time 30 min Replicate 6
Sample type
RNA
Channel 1
Source name
Cornea burn eyes, 30 min Replicate 6
Organism
Mus musculus
Extracted molecule
total RNA
Channel 2
Source name
Matched control, non burn eyes
Organism
Mus musculus
Extracted molecule
total RNA
Description
Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals. Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator. For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection. Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray. Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software. Keywords = Cornea burn
Submission date
May 11, 2004
Last update date
May 27, 2005
Contact name
Yuan Fang
E-mail(s)
[email protected]
Phone
503-494-1425
Fax
503-494-3929
Organization name
Oregon Health & Science University
Department
Ophthalmology, Casey Eye Institute
Lab
Cornea Division
Street address
3375 SW Terwilliger Blvd
City
Portland
State/province
OR
ZIP/Postal code
97239-4197
Country
USA
Platform ID
GPL1230
Series (1)
GSE1393
A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.
Data table header descriptions
ID_REF
reference key to corresponding platform
VALUE
normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag
Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean
Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean
Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean
Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean
Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median
Cy5 (F635) median fluorescence intensity
Cy5_Background_Median
Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median
Cy3 (F532) median fluorescence intensity
Cy3_Background_Median
Cy3 (F532) background median fluorescence intensity
Data table
ID_REF
VALUE
Flag
Cy5_Signal_Mean
Cy5_Background_Mean
Cy3_Signal_Mean
Cy3_Background_Mean
Cy5_Signal_Median
Cy5_Background_Median
Cy3_Signal_Median
Cy3_Background_Median
1
1.54E-01
0
272.583
203.848
314.158
263.019
182
107
191
157.5
2
-2.86E-01
0
279.118
215.376
340.996
220.611
158
114
223
131
3
4.65E-01
0
1135.54
236.884
813.444
264.683
969
120
618
168
4
-2.84E-01
0
262.973
220.558
288.423
248.988
142.5
136
167
128.5
5
-6.00E-01
0
1670.47
279.205
1912.63
247.205
1415
171
1548
148
6
3.08E-01
0
595.767
252.392
525.971
290.12
447
147
377
158
7
-8.57E-01
0
244.014
244.148
315.529
234.744
133
145.5
203
133
8
NULL
0
215.231
240.067
280.707
245.139
117
137.5
165
139
9
-7.37E-01
0
1239.27
219.236
1666.11
299.23
1024
105.5
1273
176.5
10
1.11E-01
0
220.58
191.455
265.593
269.014
117
113.5
150
169
11
-5.96E-01
0
392.71
227.783
570.24
267.719
266
116
448
146
12
8.83E-01
0
468.359
240.751
348.313
265.24
304
145
248
167
13
-1.08E+00
0
1994.1
245.382
3120.69
269.429
1571
142
2446
154
14
-2.13E-01
0
221.057
222.422
271.395
273.701
120
120.5
154
157.5
15
-5.76E-01
0
216.954
226.304
274.651
236.982
122.5
126.5
158.5
143.5
16
1.91E-01
0
228.295
212.627
267.658
281.612
125
108
153
164
17
-1.93E-01
0
380.821
214.765
448.745
258.53
234
122.5
306
149
18
-7.97E-01
0
1002.04
239.126
1386.27
276.677
761
138
1051
161.5
19
2.00E-01
0
215.311
225.796
236.707
270.8
121
112
133
150
20
-1.39E-01
0
249.084
187.463
284.691
222.11
146
109.5
174.5
124.5
Total number of rows: 16928 Table truncated, full table size 1095 Kbytes .Supplementary data files not provided