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Sample GSM22721 Query DataSets for GSM22721
Status Public on May 21, 2004
Title A chip: time 30 min Replicate 6
Sample type RNA
 
Channel 1
Source name Cornea burn eyes, 30 min Replicate 6
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name Matched control, non burn eyes
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Examine the effect of stimulation stress produced by chemical burn to the mice cornea in the lacrimal gland over a time course (0.5 - 360 hours) in comparison with the non-stimulated control animals.
Using silver nitrate applicator (silver nitrate 37.5 mg and potassium nitrate 12.5 mg, Graham-Field,Inc.,) do two burns per eye, each with a separate applicator.
For animals observed for more than 24 hours, eyes were then treted with gentamicin ointment to minimize infection.
Total RNA were extracted from both burn and control eyes and were covalently coupled separately with Cy5 and Cy3 monoreactive fluors. The Cy5 and Cy3 labelled RNA were hybridized to the microarray.
Fluorescent array images were collected for both Cy3 and Cy5 with ScanArray 4000XL and image intensity data were extracted and analyzed with Imagene analysis software.
Keywords = Cornea burn
 
Submission date May 11, 2004
Last update date May 27, 2005
Contact name Yuan Fang
E-mail(s) [email protected]
Phone 503-494-1425
Fax 503-494-3929
Organization name Oregon Health & Science University
Department Ophthalmology, Casey Eye Institute
Lab Cornea Division
Street address 3375 SW Terwilliger Blvd
City Portland
State/province OR
ZIP/Postal code 97239-4197
Country USA
 
Platform ID GPL1230
Series (1)
GSE1393 A time course microarray study of gene expression in the mouse lacrimal gland after acute corneal trauma.

Data table header descriptions
ID_REF reference key to corresponding platform
VALUE normalized ratio of the cy5 mean signal intensity/ cy3 mean signal intensity
Flag Spot flagging schemas to help remove suspicious spots from consideration. Flag = 1: Bad spots, Flag = 0: good spots"
Cy5_Signal_Mean Cy5 (F635) mean fluorescence intensity
Cy5_Background_Mean Cy5 (F635) background mean fluorescence intensity
Cy3_Signal_Mean Cy3 (F532) mean fluorescence intensity
Cy3_Background_Mean Cy3 (F532) background mean fluorescence intensity
Cy5_Signal_Median Cy5 (F635) median fluorescence intensity
Cy5_Background_Median Cy5 (F635) background median fluorescence intensity
Cy3_Signal_Median Cy3 (F532) median fluorescence intensity
Cy3_Background_Median Cy3 (F532) background median fluorescence intensity

Data table
ID_REF VALUE Flag Cy5_Signal_Mean Cy5_Background_Mean Cy3_Signal_Mean Cy3_Background_Mean Cy5_Signal_Median Cy5_Background_Median Cy3_Signal_Median Cy3_Background_Median
1 1.54E-01 0 272.583 203.848 314.158 263.019 182 107 191 157.5
2 -2.86E-01 0 279.118 215.376 340.996 220.611 158 114 223 131
3 4.65E-01 0 1135.54 236.884 813.444 264.683 969 120 618 168
4 -2.84E-01 0 262.973 220.558 288.423 248.988 142.5 136 167 128.5
5 -6.00E-01 0 1670.47 279.205 1912.63 247.205 1415 171 1548 148
6 3.08E-01 0 595.767 252.392 525.971 290.12 447 147 377 158
7 -8.57E-01 0 244.014 244.148 315.529 234.744 133 145.5 203 133
8 NULL 0 215.231 240.067 280.707 245.139 117 137.5 165 139
9 -7.37E-01 0 1239.27 219.236 1666.11 299.23 1024 105.5 1273 176.5
10 1.11E-01 0 220.58 191.455 265.593 269.014 117 113.5 150 169
11 -5.96E-01 0 392.71 227.783 570.24 267.719 266 116 448 146
12 8.83E-01 0 468.359 240.751 348.313 265.24 304 145 248 167
13 -1.08E+00 0 1994.1 245.382 3120.69 269.429 1571 142 2446 154
14 -2.13E-01 0 221.057 222.422 271.395 273.701 120 120.5 154 157.5
15 -5.76E-01 0 216.954 226.304 274.651 236.982 122.5 126.5 158.5 143.5
16 1.91E-01 0 228.295 212.627 267.658 281.612 125 108 153 164
17 -1.93E-01 0 380.821 214.765 448.745 258.53 234 122.5 306 149
18 -7.97E-01 0 1002.04 239.126 1386.27 276.677 761 138 1051 161.5
19 2.00E-01 0 215.311 225.796 236.707 270.8 121 112 133 150
20 -1.39E-01 0 249.084 187.463 284.691 222.11 146 109.5 174.5 124.5

Total number of rows: 16928

Table truncated, full table size 1095 Kbytes.




Supplementary data files not provided

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