|
| Status |
Public on Aug 04, 2016 |
| Title |
WKY_STD_biological rep 5 |
| Sample type |
RNA |
| |
|
| Source name |
WKY rats fed with standard diet
|
| Organism |
Rattus norvegicus |
| Characteristics |
gender: male
strain: WKY/NCrl
diet: standard diet
cell type: PBMC
age: 15-weeks
|
| Treatment protocol |
After an adaptation period, rats from each strain were randomly distributed into two experimental groups (n=5) and fed with either standard or cafteria diet for 7 weeks, after which blood was collected from abdominal aorta and circulating monocytes were isolated using density gradient centrifugation using Histopaque-1083
|
| Growth protocol |
Male LEW and WKY rats were housed in a 22C temperature-controlled room
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (invitrogen). Total RNA was purified using Rneasy microkits (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rattus Norvegicus Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Genechip scanner 3000-7G
|
| Description |
Gene expression data from rat PBMCs under STD or CAF diet
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 03, 2016 |
| Last update date |
Aug 04, 2016 |
| Contact name |
Jacques Behmoaras |
| E-mail(s) |
[email protected]
|
| Organization name |
Imperial College London
|
| Street address |
Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE85167 |
Expression data from rat peripheral blood mononuclear cells (PBMCs) |
|
