|
| Status |
Public on Mar 28, 2017 |
| Title |
CHOW EPI 2 Hi |
| Sample type |
RNA |
| |
|
| Source name |
mouse PDGFRα+ cells with high CD9 expression (CD9high)
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C3H
phenotype: lean
age: 3 months
tissue: epididymal white adipose tissue (EpiWAT)
cell type: CD45- CD31- Gp38+ PDGFR-alpha-plus CD9high
|
| Treatment protocol |
EpiWAT from 3-month-old C3H mice was digested with collagenase. CD9high and CD9low progenitors from the stromal vascular fraction were FACS-sorted with CD45- CD31- Gp38+ PDGFRa+ CD9high and CD45- CD31- Gp38+ PDGFRa+ CD9high.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified and labeled with biotin using Ovation Pico WTA System V2 (NuGEN) and Encore BiotinIL Module (NuGEN) kits according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
SAMPLE 3
101036410004_C
|
| Data processing |
The data were normalised using quantile normalisation and background subtracted with GenomeStudio (Illumina software).
|
| |
|
| Submission date |
Jul 25, 2016 |
| Last update date |
Mar 28, 2017 |
| Contact name |
veronique PELLOUX |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM
|
| Department |
UMR 1166 eq 6
|
| Lab |
Hopital Pitié-Salpêtrière -E3M
|
| Street address |
50-52 bld Vincent Auriol
|
| City |
paris |
| ZIP/Postal code |
75013 |
| Country |
France |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE84822 |
Microarray analysis of CD9high and CD9low progenitors isolated from epididymal adipose from lean C3H/HeOuJ mice |
| GSE84823 |
Microarray analysis of CD9high and CD9low progenitors isolated from adipose tissue |
|
