|
| Status |
Public on Jul 23, 2016 |
| Title |
Wild Type base crypt cells 3 |
| Sample type |
RNA |
| |
|
| Source name |
epithelial cells from the base of the colonic crypt of wild-type mouse
|
| Organism |
Mus musculus |
| Characteristics |
age: 10-12 weeks
strain: C57BL/6
cell population: crypt-base
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed with the Arcturus PicoPure RNA extraction kit (Applied Biosystems/Life Technologies) according to manufucturer's protocols, treated with DNase (Qiagen, Valencia, CA), and amplified twice using a Nugen WT-Ovation kit (San Carlos, CA). RNA integrity was confirmed by Agilent 2100 Bioanayzer (Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation and log2 transformation with Illumina Genome Studio
|
| |
|
| Submission date |
Jul 22, 2016 |
| Last update date |
Jul 23, 2016 |
| Contact name |
Loukia Lili |
| E-mail(s) |
[email protected]
|
| Phone |
4046422896
|
| Organization name |
The Institute for Next Generation Healthcare
|
| Department |
Icahn School of Medicine at Mount Sinai
|
| Street address |
770 Lexington Ave, 14-07 14th floor
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE84742 |
Claudin-based barrier differentiation in the colonic epithelial crypt niche involves Hopx/Klf4 and Tcf7l2/Hnf4-alpha cascades |
|
