|
| Status |
Public on Feb 10, 2017 |
| Title |
JC3 |
| Sample type |
RNA |
| |
|
| Source name |
CD34+ isolated from human blood sample
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: megakaryocytes
disease state: control
|
| Treatment protocol |
The cells were not treated in any way
|
| Growth protocol |
Cells were maintained in IMDM supplemented with 100 U /ml penicillin/streptomycin, 2 mM l-glutamine, 76nM α-monothioglycerol, 1.5% BSA, 20 μg/ml lipids, insulin transferrin selenium and 10ng/mL of recombinant human thrombopoietin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using RNeasy Micro Kit (Qiagen) according to manufacturer's instructions. The quantity and quality of the isolated RNAs was determined using ND-1000 Spectrophotometer.
|
| Label |
none
|
| Label protocol |
Biotinylated, amplified RNA for hybridization with the Illumina HT12v4 arrays was prepared using the Illumina® TotalPrep™ RNA Amplification Kit (Ambion, Cat. no: AMIL1791)
|
| |
|
| Hybridization protocol |
The hybridization, washing and staining of the arrays was performed according to Illumina provided protocols.
|
| Scan protocol |
The arrays were scanned using the Illumina HiScan or BeadArray Reader (Cleveland)
|
| Description |
JC3_Control 2 41+42-
SAMPLE 3
|
| Data processing |
The data were processed and normalised using Illumina GenomeStudio
|
| |
|
| Submission date |
Jul 20, 2016 |
| Last update date |
Feb 10, 2017 |
| Contact name |
John Crispino |
| E-mail(s) |
[email protected]
|
| Organization name |
Northwestern University
|
| Department |
Medicine-Hematology/Oncology
|
| Lab |
Robert H Lurie Medical Research Center Room 5-250
|
| Street address |
303 E Superior
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE84638 |
Comparison of gene expression in megakaryocytes derived from primary myelofibrosis or healthy individuals |
|
