|
| Status |
Public on May 22, 2017 |
| Title |
BAY1436032 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
sorted human CD45+ cells from bone marrow of PDX mouse treated with BAY1436032
|
| Organism |
Homo sapiens |
| Characteristics |
pdx mouse: NSG mouse transplanted with human IDH1mut AML patient cells
treatment: BAY1436032
cell type: sorted human CD45+ cells from bone marrow of PDX mouse
|
| Treatment protocol |
Human IDH1mut AML patient cells were transplanted in NSG mice. Mice were treated with either vehicle or 150mg/kg/day of BAY1436032. Four weeks post treatment, bone marrow was isolated and human CD45+ positive cells were sorted for RNA preparation.
|
| Growth protocol |
in vivo from NSG mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Plus Mini Kit according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA were used for biotin labelling according to the 3' IVT Express Kit (Affymetrix; Santa Clara, CA).
|
| |
|
| Hybridization protocol |
7.5 µg of biotinylated cRNA were fragmented and placed in a hybridization cocktail containing four biotinylated hybridization controls (BioB, BioC, BioD, and Cre) as recommended by the manufacturer. Samples were hybridized to an identical lot of Affymetrix GeneChip HG-U133 2.0 for 16 hours at 45°C. Steps for washing and SA-PE staining were processed on the Fluidics Station 450 using the recommended FS450 protocol (Affymetrix; Santa Clara, CA).
|
| Scan protocol |
Image analysis was performed on GCS3000 Scanner and GCOS1.2 Software Suite (Affymetrix; Santa Clara, CA).
|
| Description |
Gene expression data from IDH1mut PDX mice treated with BAY1436032.
|
| Data processing |
Analysis of microarray data was performed using GeneSpring 11.5.1 software suite (Agilent Technologies, CA, USA). Signal intensities (raw data) were background corrected, normalized and summarized using RMA. Gene expression values per condition are given as log2 relative expression to mean expression value calculated from log2 intensities of all conditions (mean centralization, normalized data). Significance of differential gene expression was calculated by GeneSpring software using error corrected unpaired T-Test.
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| |
|
| Submission date |
Jun 17, 2016 |
| Last update date |
May 23, 2017 |
| Contact name |
Anuhar Chaturvedi |
| E-mail(s) |
[email protected]
|
| Phone |
00495115329778
|
| Organization name |
Hannover Medical School
|
| Department |
Hematology, Hemostasis, Oncology and Stem Cell Transplantation
|
| Lab |
AG Heuser
|
| Street address |
Cral Neuberg Strasse-1
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE83485 |
Gene expression changes induced by BAY1436032 (IDH1mut inhibitor) in sorted human (CD45+) cells from bone marrow of IDH1mut patient derived xenotransplantation mice model |
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