|
Status |
Public on Nov 23, 2020 |
Title |
SAMPLE 10: WT_Sham |
Sample type |
RNA |
|
|
Source name |
WT_Sham
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J treatment: SHAM genotype: WT-Littermate tissue: Cardiac tissue
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with TriReagent ( Sigma). Quality control ( RQI) was performed with Biorad Experion.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit ( Applied Biosystems)
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol using the Illumina iScan
|
Description |
9343563049_B
|
Data processing |
The Data where normalized using quantile normalisation strategies improved with a batch controlling Combat algorithm, PMID 20181233
|
|
|
Submission date |
Jun 17, 2016 |
Last update date |
Nov 23, 2020 |
Contact name |
vincent bloks |
Organization name |
UMCG
|
Department |
Pediatrics
|
Lab |
Pediatrics
|
Street address |
Hanzeplein1
|
City |
Groningen |
ZIP/Postal code |
9713GZ |
Country |
Netherlands |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE83472 |
Overexpression of A kinase interacting protein 1 attenuates myocardial ischemia / reperfusion injury, but does not influence heart failure development |
|