|
| Status |
Public on Jun 09, 2016 |
| Title |
M226-14L |
| Sample type |
RNA |
| |
|
| Source name |
Adenocarcinoma
|
| Organism |
Homo sapiens |
| Characteristics |
patient no: 4
cancer: Colorectal cancer
1st line treatment: oxa
1st line best response: Non-Responder
|
| Treatment protocol |
Metastatic colorectal cancer patients (stage IV) treated first-line with 5FU and oxaliplatin (described in DOI: 10.1016/j.molonc.2013.02.016)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cancer or adjacent normal epithelium cells (as appropriate) were laser-microdissected (Veritas 704) from snap frozen tissue biopsies, and total RNA extracted using the RNeasy micro kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Total RNA (~10-50 ng) were amplified, fragmented and biotin labelled according to manufacturer's reccomendation (Pico Ovation kit , Nugen)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix standard hybridization kit materials, and chips washed on a Fluidics 450 apparatus
|
| Scan protocol |
Affymetrix Gene ChIP 3000 7G Scanner
|
| Description |
Total RNA were subjected to amplification (Pico Ovation kit , Nugen)
|
| Data processing |
Data passed QC control metrices in Affymetrix Gene Expression Console. CEL files were loaded into GeneSpring v.12.5 were probes intensities were quantile normalized and summarized using IterPLIER.
probe group file: HuEx-1_0-st-v2.r2.pgf
meta-probeset file: HuEx-1_0-st-v2.r2.dt1.hg18.full.mps
|
| |
|
| Submission date |
Jun 08, 2016 |
| Last update date |
Jun 09, 2016 |
| Contact name |
mads heilskov rasmussen |
| E-mail(s) |
[email protected]
|
| Organization name |
Aarhus University Hospital
|
| Street address |
Brendstrupgårdsvej 21
|
| City |
Aarhus |
| State/province |
Please Select |
| ZIP/Postal code |
8220 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE83129 |
RNA profiling in metastatic colorectal cancer patients treated first-line with oxaliplatin |
|
