|
| Status |
Public on Aug 01, 2016 |
| Title |
SD28 |
| Sample type |
RNA |
| |
|
| Source name |
SD28 middle lobe
|
| Organism |
Homo sapiens |
| Characteristics |
patient reference: SSc-C
tissue: lung
diagnosis: Systemic Sclerosis with interstitial lung disease (SSc-ILD)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated using microRNeasy Mini Kits Qiagen protocol
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled amplified total RNA was purified, fragmented, and hybridized to Affymetrix U133A 2.0 microarrays.
|
| |
|
| Hybridization protocol |
Not provided
|
| Scan protocol |
The signals of the samples were amplified and microarrays were immediately scanned using Affymetrix GeneArray Scanner 3000 7G Plus (Affymetrix Santa Clara, CA).
|
| Data processing |
The resulting CEL files were summarized using Affymetrix Expression Console (current version 1.1). The MAS5 algorithm with global scaling normalization was used to generate gene-level expression data.
|
| |
|
| Submission date |
May 10, 2016 |
| Last update date |
Aug 01, 2016 |
| Contact name |
Romy B Christmann |
| E-mail(s) |
[email protected]
|
| Phone |
6176384309
|
| Organization name |
Boston University School of Medicine
|
| Street address |
715 Albany Street E5
|
| City |
Boston |
| State/province |
MASSACHUSETTS |
| ZIP/Postal code |
02118-2531 |
| Country |
USA |
| |
|
| Platform ID |
GPL18991 |
| Series (2) |
| GSE81292 |
Expression of mRNA from lung tissue from Systemic Sclerosis patients with interstitial lung disease (SSc-ILD) and healthy controls (HC) |
| GSE81294 |
Lung gene expression |
|
