|
| Status |
Public on Apr 19, 2016 |
| Title |
Airway stem cell, P8 replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Bronchus
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: airway stem cells
passage: 8
|
| Growth protocol |
the cells are cultured in SAGM + 5-10 micro-molar ROCKi, 1 micro-molar A-83-01, 1 micro-molar DMH-1, 1 micro-molar CHIR99021 and expanded to P2, P8 and P14 (1:10 splitting ratio)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA is isolated from human airway stem cells from early to late passages using TRIzol (Invitrogen) and the miRNeasy mini kit (Qiagen) according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Standard protocol
|
| |
|
| Hybridization protocol |
RNA is hybridized on Illumina Human HT-12 (Translational Genomics Core, MGH), standard protocol
|
| Scan protocol |
Data is scaned in Translational Genomics Core, MGH using Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
quantile normalization
|
| |
|
| Submission date |
Apr 18, 2016 |
| Last update date |
Apr 19, 2016 |
| Contact name |
Hongmei Mou |
| E-mail(s) |
[email protected]
|
| Organization name |
Massachusetts General Hospital
|
| Department |
The Mucosal Immunology & Biology Research Center
|
| Lab |
Hongmei Mou
|
| Street address |
55 Fruit Street, Jackson 1402
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE80408 |
Transcriptome analysis of human airway stem cells at their early and late passages expanded in dual SMAD signaling inhibitors |
|
