|
| Status |
Public on Dec 02, 2016 |
| Title |
HDFa treated with PBS control, biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
HDFa cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HDFa
cell type: fibroblasts
|
| Treatment protocol |
Cells were treated with either 8µg/mL ES isolated from instar 1 lucilia sericata larvae in PBS or an equal volume of PBS
|
| Growth protocol |
Cells grown in vitro with appropriate media, either DMEM, RPMI or MCDB 131 supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the Ambion mirvana kit with the protocol for total RNA
|
| Label |
biotin
|
| Label protocol |
biotinylated cRNA was made from 200ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression in fibroblasts treated with control buffer
|
| Data processing |
Data was analysed using Affymetrix software Gene expresison console and normalised in cell-line batched with RMA and background correction
|
| |
|
| Submission date |
Mar 31, 2016 |
| Last update date |
Dec 02, 2016 |
| Contact name |
Cherie Blenkiron |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Auckland
|
| Department |
Surgery
|
| Lab |
ASML
|
| Street address |
Private bag 92019
|
| City |
Auckland |
| ZIP/Postal code |
1142 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE79765 |
Effects of maggot excretions and secretions (ES) on human cultured cells in vitro |
|
