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Sample GSM2102114 Query DataSets for GSM2102114
Status Public on Dec 02, 2016
Title HMEC-1 treated with 8µg/mL maggot ES, biological replicate 3
Sample type RNA
 
Source name HMEC-1 cell line
Organism Homo sapiens
Characteristics cell line: HMEC-1
cell type: endothelial cell
Treatment protocol Cells were treated with either 8µg/mL ES isolated from instar 1 lucilia sericata larvae in PBS or an equal volume of PBS
Growth protocol Cells grown in vitro with appropriate media, either DMEM, RPMI or MCDB 131 supplemented with 10% FBS
Extracted molecule total RNA
Extraction protocol RNA was isolated using the Ambion mirvana kit with the protocol for total RNA
Label biotin
Label protocol biotinylated cRNA was made from 200ng total RNA
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression in endothelial cells treated with maggot ES
Data processing Data was analysed using Affymetrix software Gene expresison console and normalised in cell-line batched with RMA and background correction
 
Submission date Mar 31, 2016
Last update date Dec 02, 2016
Contact name Cherie Blenkiron
E-mail(s) [email protected]
Organization name University of Auckland
Department Surgery
Lab ASML
Street address Private bag 92019
City Auckland
ZIP/Postal code 1142
Country New Zealand
 
Platform ID GPL6244
Series (1)
GSE79765 Effects of maggot excretions and secretions (ES) on human cultured cells in vitro

Data table header descriptions
ID_REF
VALUE RMA normalised data

Data table
ID_REF VALUE
7892501 4.5
7892502 3.45
7892503 3.35
7892504 7.9
7892505 2.95
7892506 4.07
7892507 6.46
7892508 2.31
7892509 11.82
7892510 4.21
7892511 4.64
7892512 6.58
7892513 3.57
7892514 10.28
7892515 9.91
7892516 7.45
7892517 4.52
7892518 2.64
7892519 4.03
7892520 8.5

Total number of rows: 33297

Table truncated, full table size 420 Kbytes.




Supplementary file Size Download File type/resource
GSM2102114_HMECES3.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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