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Status |
Public on Dec 02, 2016 |
Title |
HMEC-1 treated with 8µg/mL maggot ES, biological replicate 3 |
Sample type |
RNA |
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|
Source name |
HMEC-1 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: HMEC-1 cell type: endothelial cell
|
Treatment protocol |
Cells were treated with either 8µg/mL ES isolated from instar 1 lucilia sericata larvae in PBS or an equal volume of PBS
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Growth protocol |
Cells grown in vitro with appropriate media, either DMEM, RPMI or MCDB 131 supplemented with 10% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using the Ambion mirvana kit with the protocol for total RNA
|
Label |
biotin
|
Label protocol |
biotinylated cRNA was made from 200ng total RNA
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|
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Description |
Gene expression in endothelial cells treated with maggot ES
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Data processing |
Data was analysed using Affymetrix software Gene expresison console and normalised in cell-line batched with RMA and background correction
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Submission date |
Mar 31, 2016 |
Last update date |
Dec 02, 2016 |
Contact name |
Cherie Blenkiron |
E-mail(s) |
[email protected]
|
Organization name |
University of Auckland
|
Department |
Surgery
|
Lab |
ASML
|
Street address |
Private bag 92019
|
City |
Auckland |
ZIP/Postal code |
1142 |
Country |
New Zealand |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE79765 |
Effects of maggot excretions and secretions (ES) on human cultured cells in vitro |
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