|
| Status |
Public on Mar 10, 2018 |
| Title |
Lung_CD11c_cre+_MyD88 fx_2h_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Lung_CD11c_2h
|
| Organism |
Mus musculus |
| Characteristics |
background: C57BL/6
gender: male
tissue: Lung
timepoint: 2h
treatment: OVA/ stFLA
genotype: MyD88 fx/fx; Itgax-cre+/Tg
|
| Treatment protocol |
Mice were either untreated or sensitized oropharyngeally with 100 ug ovalbumin (OVA)/ 1250 ng standard flagellin (stFLA) for 2 or 6 hours in vivo. After this time, lungs were isolated and snap frozen for RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Frozen lung lobes were homogenized and RNA isolated from whole lungs using the Qiagen Rneasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
50 ng of each total RNA sample was labeled according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridization was done according to the manufacturer's instructions.
|
| Scan protocol |
Gene expression was quantified on the nCounter Digital AnalyzerTM.
|
| Description |
Gene expression from MyD88 fx/fx; Itgax-cre+/Tg lung treated with OVA/ stFLA for 2h.
|
| Data processing |
Raw data was normalized using the Nanostring nCounter software.
|
| |
|
| Submission date |
Mar 24, 2016 |
| Last update date |
Mar 10, 2018 |
| Contact name |
Seddon Thomas |
| E-mail(s) |
[email protected]
|
| Organization name |
NIEHS
|
| Street address |
111 T. W. Alexander Dr.,
|
| City |
RTP |
| State/province |
North Carolina |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL19964 |
| Series (2) |
| GSE79594 |
Nanostring of whole lung RNA from cell-specific MyD88 KO at 0h, 2h and 6h after in vivo sensitization with OVA/standard flagellin |
| GSE79615 |
MYD88-dependent dendritic and epithelial cell crosstalk in the lung orchestrates immune responses to inhaled allergens |
|
