|
| Status |
Public on Nov 22, 2018 |
| Title |
Postveraison berry (12_14 weeks post flowering), 9 hours after heat shock (HSPpos010_06.t5.r2) |
| Sample type |
RNA |
| |
|
| Source name |
postveraison berry (12_14 weeks post flowering), 9 hours after heat shock
|
| Organism |
Vitis vinifera |
| Characteristics |
Cabernet Sauvignon
|
| Treatment protocol |
Plants were acclimatized for 48hrs 16oC day/night for a 16hr day. Plants were moved to a growth chamber set at 38oC for heat shock treatment.
|
| Growth protocol |
Berries were collected from bunches from plants grown in a growth cabinet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The modified RNAeasy RNA extraction method (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Affymetrix One Cycle cDNA synthesis kit, Affymetrix GeneChip Sample Cleanup kit, Affymetrix IVT labeling kit
|
| |
|
| Hybridization protocol |
Affymetrix Fluidics Station 450, Biotin/Streptavidin detection
|
| Scan protocol |
The GeneChips were scanned using theGeneChip Scanner 3000
|
| Description |
deseeded, ripe (postverasion) berries grown under laboratory conditions in a growth cabinet, sampled 9 hours post heat shock treatment
|
| Data processing |
Data normalized using the GCRMA R package from Bioconductor
|
| |
|
| Submission date |
Jul 05, 2007 |
| Last update date |
Nov 22, 2018 |
| Contact name |
Mark Raymond Thomas |
| E-mail(s) |
[email protected]
|
| Organization name |
CSIRO
|
| Department |
Plant Industry
|
| Street address |
PO Box 350
|
| City |
Glen Osmond |
| State/province |
SA |
| ZIP/Postal code |
5064 |
| Country |
Australia |
| |
|
| Platform ID |
GPL1320 |
| Series (1) |
| GSE8389 |
The effects of heat shock treatment on gene expression of grapevine berries (HSP_06) |
|
