|
| Status |
Public on May 12, 2016 |
| Title |
44G: 24 month old gastrocnemius muscle rep4 |
| Sample type |
SRA |
| |
|
| Source name |
44G
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
gender: male
tissue: gastrocnemius muscle
age (months): 24
animal id: 44
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
mRNA was extracted using the miRNeasy kit from Qiagen, following the manufacturer's instructions.
TruSeq RNA Library Preparation Kit v2
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina Casava 1.8 software used for basecalling and demultiplexing.
Sequenced reads were trimmed for adaptor sequence, and mapped to Rnor 6.0 whole genome using RNA-STAR v2.3.0e with parameters --genomeLoad NoSharedMemory --runThreadN 12
Counts within genes were generated using HTSeq, mode union, strand no, annotation file Rattus_norvegicus.Rnor_6.0.80.gtf
Genome_build: RGSC Rnor_6.0
Supplementary_files_format_and_content: tab-delimited text file including counts per gene for each sample quantified by HTSeq
|
| |
|
| Submission date |
Feb 26, 2016 |
| Last update date |
Mar 13, 2020 |
| Contact name |
Jerome Feige |
| Organization name |
Nestle Institute Of Health Sciences
|
| Department |
Musculo-Skeletal Health
|
| Street address |
EPFL Innovation Park, Building H
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL18694 |
| Series (2) |
| GSE78702 |
Comparison of age-related changes in gene expression of 2 rat skeletal muscles (8 months, 18 months and 24 months) |
| GSE146977 |
Comparison of age-related changes in gene expression of 2 rat skeletal muscles |
|
| Relations |
| BioSample |
SAMN04517693 |
| SRA |
SRX1602146 |
