|
| Status |
Public on Feb 23, 2017 |
| Title |
P72L |
| Sample type |
SRA |
| |
|
| Source name |
cerebral ischemia_left_brain tissue
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: male
time point: after 24 hrs of middle cerebral artery occlusion (MCAO)
tissue: brain tissue
side: Left
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extract by standard TriZol protocol.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Clean reads were aligning to rn5 with TopHat2 (Kim et al., 2013). Gene expression levels were estimated by Cufflinks (Ghosh and Chan 2016). Genome DNA sequence and gene annotation were download from UCSC genome browser.
Genome_build: rn5
Supplementary_files_format_and_content: BED format file that annotate novel lncRNAs. Tab-delimited text files include FPKM values for each Sample.
|
| |
|
| Submission date |
Feb 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Kunshan Zhang |
| E-mail(s) |
[email protected]
|
| Organization name |
Tongji Hospital, Tongji University
|
| Department |
Stem cell Translational Research center
|
| Street address |
389 Xincun Road
|
| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
| |
|
| Platform ID |
GPL18694 |
| Series (1) |
| GSE78200 |
Transcriptome analyses of cerebral ischemia reveal functional lncRNAs in ischemia stroke |
|
| Relations |
| BioSample |
SAMN04510345 |
| SRA |
SRX1597419 |
