|
| Status |
Public on Mar 27, 2008 |
| Title |
Wild type, chitooctaose treatment, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Two-week-old whole seedlings of Arabidopsis thaliana
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Genotype: WT Col-0
Two-week-old seedlings
|
| Treatment protocol |
Seedlings grown hydroponically were treated wtih chitooctaose at a final concentration of 1 um for 30 minutes. The collected seedlings were frozen in liquid nitrogen for RNA isolation uisng the Trizol solution (Invitrogen).
|
| Growth protocol |
Seedlings were grown in the MS liquid medium for 14 days under constant light at 23 degree and then treated with chitooctaose
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized with the Affymetrix Arabidopsis Whole Genome Array ATH1 according to the manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned using a Hewlett-Packard GeneArray Scanner
|
| Description |
Gene expression in response to chitooctaose
|
| Data processing |
The data were analyzed with DNA-CHIP (dChip) (Version, September 23, 2005) using the default settings.
|
| |
|
| Submission date |
Jun 28, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Gary Stacey |
| E-mail(s) |
[email protected]
|
| Phone |
573-884-4799
|
| URL |
http://psu.missouri.edu/staceylab/index.htm
|
| Organization name |
University of Missouri-Columbia
|
| Department |
Plant Sciences
|
| Street address |
1201 Rollins Road
|
| City |
Columbia |
| State/province |
MO |
| ZIP/Postal code |
65211 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE8319 |
A LysM Receptor-like Kinase Mediates Chitin Perception and Fungal Resistance in Arabidopsis |
|
| Relations |
| Reanalyzed by |
GSE118579 |
| Reanalyzed by |
GSE119083 |
