|
| Status |
Public on Nov 10, 2007 |
| Title |
GOLD-0_Pool_n=8 vs GOLD-2__3388 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Pooled Lung Samples
|
| Organism |
Homo sapiens |
| Characteristics |
GOLD-0_Pool_n=8
|
| Treatment protocol |
Lung samples from COPD patients
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Lysis + RNeasy Column
|
| Label |
Cy3
|
| Label protocol |
One round of RT-IVT to cRNA
|
| |
|
| Channel 2 |
| Source name |
Lung samples from COPD patient
|
| Organism |
Homo sapiens |
| Characteristics |
GOLD-2__3388
|
| Treatment protocol |
Lung samples from COPD patients
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Lysis + RNeasy Column
|
| Label |
Cy5
|
| Label protocol |
One round of RT-IVT to cRNA
|
| |
|
| |
| Hybridization protocol |
Labeled cRNAs were fragmented to an average size of approximately 50 to 100 nucleotides in the presence of 10 mM zinc chloride and adding a hybridization buffer containing 1 M sodium chloride, 0.5 percent sodium sarcosine, 50 mM morpholino-ethane sulfonic acid (pH 6.5), and formamide (final concentration, 30 percent at 40°C); After hybridization, the slides were washed
|
| Scan protocol |
slides were scanned using a confocal laser scanner (Agilent Technologies, Palo Alto, CA). Fluorescence intensities of the scanned images were quantified, normalized, and balanced
|
| Description |
Lung profiling from COPD patients
|
| Data processing |
Rosetta Resolver Ratio Experiment using a pair of Fluor-Reversed samples normalised using the Rosetta Resolver Agilent Error Model
|
| |
|
| Submission date |
Jun 27, 2007 |
| Last update date |
Oct 29, 2007 |
| Contact name |
serguei b stepaniants |
| Organization name |
Merck
|
| Department |
Rosetta
|
| Lab |
Informatics
|
| Street address |
401 Terry Ave. N
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL3991 |
| Series (1) |
| GSE8500 |
Gene Expression Profiling in the Lungs of Patients with Chronic Obstructive Pulmonary Disease (COPD) and Lung Cancer |
|
