|
| Status |
Public on Jan 01, 2017 |
| Title |
SScMH_29_Base_LF |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Sample: Human Tissue Biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skin biopsy
subject: SScMH_29
disease state: SSc patient
treatment: MTX
|
| Treatment protocol |
Storage of human tissue in RNAlater
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Fibrous Tissue Mini following manufacturers protocol
|
| Label |
Cy3
|
| Label protocol |
200ng of total RNA was reversed transcribed using Agilent Quick-Amp Labeling Kit and labeled with Perkin Elmer Cy3
|
| |
|
| Channel 2 |
| Source name |
Universal Human Reference
|
| Organism |
Homo sapiens |
| Characteristics |
reference: Universal Human Reference
|
| Treatment protocol |
Storage of human tissue in RNAlater
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Fibrous Tissue Mini following manufacturers protocol
|
| Label |
Cy5
|
| Label protocol |
200ng of total RNA was reversed transcribed using Agilent Quick-Amp Labeling Kit and labeled with Perkin Elmer Cy3
|
| |
|
| |
| Hybridization protocol |
Fragmentation Buffer and Hybridization Buffer (Agilent Gene Expression Hybridization Kit) were added, and samples applied to Whole Human Genome microarrays
|
| Scan protocol |
Scanned on Axon scanner
|
| Description |
SSc patient
|
| Data processing |
Images were quantified using GenePixPro
|
| |
|
| Submission date |
Jan 14, 2016 |
| Last update date |
Jan 01, 2017 |
| Contact name |
Tammara Wood |
| E-mail(s) |
[email protected]
|
| Organization name |
Dartmouth Medical School
|
| Department |
Biomedical Data Science
|
| Lab |
Whitfield Lab
|
| Street address |
WTRB 674
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (2) |
| GSE76885 |
Systemic sclerosis skin disease trajectory during mycophenolate mofetil treatment |
| GSE76886 |
Hinchcliff 359 microarray dataset |
|
