Selected by FACS for positive staining for prominin and GFP
Extracted molecule
total RNA
Extraction protocol
RNeasy Mini kit (Qiagen) with on column DNase treatment
Label
Biotin
Label protocol
MessageAmp II-Biotin Enhanced (Ambion, 1791)
Hybridization protocol
Affymetrix Expression protocol
Scan protocol
Affymetrix Expression protocol
Description
Prospective isolation of radial glia subtypes – lineage and transcriptom analysis Luisa Pinto1, Michael Mader1, Martin Irmler2, Marco Gentilini3, Federico Santoni4, Vassiliki Nikoletopoulou5, Steven Pollard6, Alessandro Bulfone3, Yves Barde5, Johannes Beckers2, and Magdalena Götz1,7,8