|
| Status |
Public on Jun 13, 2017 |
| Title |
MCF7-shEP2, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 cell stably transfected with lentiviral vector containing a shEP300 construct targeting EP300
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell type: epithelial breast cancer cell line
transfected with: lentiviral vector containing a shEP300 construct targeting EP300
genotype/phenotype: containing shEP2, another hairpin targeting human EP300
|
| Growth protocol |
All the EP300 knocked-down and their corresponding paclitaxel resistant derivatives MCF7 cells were grown in Dulbecco's Modified Eagle's Medium (DMEM) containing fetal calf serum (FCS) and glutamine.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extractions were performed using RNeasy Mini Kit from QIAGEN and according to the manufacturer's instruction. A post-extraction QC checking of the RNA sample integrity was performed by measuring RNA Integrity Number (RIN) using a bioanalyser (Agilent 2100, DE24802458, ver. 2.6).
|
| Label |
biotin
|
| Label protocol |
Biotinylated single-stranded cDNA (ss-cDNA) samples were prepared according to the standard Affymetrix protocol from 5.5 µg ss-cDNA (User Guide for GeneChip® WT PLUS Reagent Kit, Manual Target Preparation for GeneChip® Whole Transcript (WT) Expression Arrays (P/N 703147 Rev. 3, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation and labeling, 3.5 µg of ss-cDNA were hybridized for 16 hr at 45°C on GeneChip® Cartridge Array. GeneChips were washed and stained in the GeneChip® Fluidics Station 450 (Affymetrix, P/N 00-0079).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
shEP2_2
|
| Data processing |
The data were analyzed with Partek Genomics Suite version 6.6 using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 21, 2015 |
| Last update date |
Jun 13, 2017 |
| Contact name |
Patricia Sancho |
| E-mail(s) |
[email protected]
|
| Organization name |
Hospital Miguel Servet IIS Aragón
|
| Department |
Traslational Research Unit
|
| Lab |
Metabolism & Cancer Stem Cells
|
| Street address |
P. Isabel la Católica 1-3
|
| City |
Zaragoza |
| State/province |
Aragón |
| ZIP/Postal code |
50009 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE76200 |
Gene Expression Pattern in EP300 knocked-down MCF7 cells and corresponding paclitaxel resistant derivatives |
|
