|
| Status |
Public on Dec 10, 2015 |
| Title |
Naïve_CD8+ CDC |
| Sample type |
RNA |
| |
|
| Source name |
spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57bl/6
cell type: Naïve CD8+ CDC
age: 6-8 week adult
gender: female
|
| Treatment protocol |
Splenic mononuclear cells were enriched by negative magnetic bead depletion of T and B cells. Enriched cells were then FACS purified for pDC, CD11b+ cDC, and CD8+ cDC by BD FACS ARIA sort
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy mini kits were used for isolation of total RNA
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA amplified (2-Target Labeling Assay) from 20ng total RNA were prepared according to the standard Affymetrix protocol available (Technical Manual Affymetrix 2006)
|
| |
|
| Hybridization protocol |
Following fragmentation, 15ug cRNA were hybridized to mouse genechip 430 2.0 for 16hr 45C
|
| Scan protocol |
Genechips were scanned using the GCS3000 scanner
|
| Description |
pooled from 5-8 mice/group
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array where MAS5 has been median scaled to value of 100
|
| |
|
| Submission date |
Dec 09, 2015 |
| Last update date |
Dec 10, 2015 |
| Contact name |
Monica Macal |
| Organization name |
UC San Diego
|
| Lab |
Elina Zuniga Lab
|
| Street address |
9500 Gilman Dr 0322
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0322 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE75834 |
Gene expression profile comparison of DC subsets |
|
