|
| Status |
Public on Dec 15, 2015 |
| Title |
embryo at E12.5, Hand2 mutant, head |
| Sample type |
RNA |
| |
|
| Source name |
mouse Hand2 mutant embryos at E12.5
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Hand2 mutant (Hand2 CAT/+; Wnt1-Cre)
tissue: head
developmental stage: E12.5
background strain: mixed C57BL/6J x ICR
|
| Treatment protocol |
Embryos of the appropriate stage were manually selected under the dissecting scope. Selected embryos were transferred to a basket, rinsed with PBS and placed on ice in the Trizol solution (GibcoBRL).
|
| Growth protocol |
Wild-type and Hand2 mutant (Hand2 CAT/+; Wnt1-Cre) embryos were collected at E11.5 or E12.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol (Invitrogen) and Rneasy Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3'IVT Express Kit User Manual, P/N 702646 Rev.1 Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner3000 (Affymetrix 00-0074).
|
| Description |
Gene expression data from E12. 5 Hand2 mutant embryos
|
| Data processing |
The data were analyzed with GeneChip Operating Software ver1.4 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 08, 2015 |
| Last update date |
Dec 15, 2015 |
| Contact name |
Noriko Funato |
| E-mail(s) |
[email protected]
|
| Phone |
+81358035796
|
| Organization name |
Tokyo Medical and Dental University
|
| Street address |
1-5-45 Yushima
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1138510 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE75805 |
Expression data from Hand2 mutant mouse embryos |
|
