|
| Status |
Public on Jun 01, 2016 |
| Title |
Plasmid_replicate_5_7.5k_Pool |
| Sample type |
SRA |
| |
|
| Source name |
plasmid DNA
|
| Organism |
synthetic construct |
| Characteristics |
cell line: Plasmid DNA
|
| Growth protocol |
24 hour growth post-transfection
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Qiagen Rneasy followed by hybridization pulldown of the GFP transcript.
Illumina Custom Paired-end (TruSeq)
|
| |
|
| Library strategy |
OTHER |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
7.5k Oligo Pool
barcode count data
fastq of 3' UTR barcode and residual 5' flanking sequencing
|
| Data processing |
Library strategy: MPRA
Perfect UTR barcode matches were asigned to their respective oligo. Counts for all barcodes asigned to an oligo were summed.
Genome_build: hg19
|
| |
|
| Submission date |
Dec 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Ryan Tewhey |
| Organization name |
Jackson Laboratory
|
| Street address |
600 Main Street
|
| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
| |
|
| Platform ID |
GPL19604 |
| Series (1) |
| GSE75661 |
Direct identification of hundreds of expression-modulating variants using a multiplexed reporter assay |
|
| Relations |
| BioSample |
SAMN04313665 |
| SRA |
SRX1462163 |
