|
| Status |
Public on Jan 09, 2017 |
| Title |
CA-0_2 |
| Sample type |
RNA |
| |
|
| Source name |
Cabernet Sauvignon berries collected at time point T0 of the post-harvest withering process, replicate 2
|
| Organism |
Vitis vinifera |
| Characteristics |
tissue: Berries (pericarp)
sampling time points (days after harvest = t0): time point T0, 0 days after harvest
% weight compared to harvest: 100%
cultivar: Vitis Vinifera cv Cabernet Sauvignon
|
| Treatment protocol |
Grape berries harvested at full maturity were placed in barns under controlled conditions to undergo post-harvest withering process.
|
| Growth protocol |
Grape berries were harvested at full maturity during vintage 2010 in a commercial vineyard located in the province of Verona.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from ~200 mg of berry (pericarp) using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, MO) with some modifications as reported in Fasoli, et al. (2012). RNA quality and quantity were assessed using a Nanodrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE) and a Bioanalyzer Chip RNA 7500 series II (Agilent, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
cDNA synthesis and labeling reactions were performed according to the NimbleGen Arrays User’s Guide (V 3.2).
|
| |
|
| Hybridization protocol |
Hybridization and washing procedures were performed according to the NimbleGen Arrays User’s Guide (V 3.2).
|
| Scan protocol |
Each microarray was scanned using a Axon GenePix 4400 A at 532 nm (Cy-3 absorption peak) and GenePix Pro7 software (Molecular Devices, Sunnyvale, CA, USA) according to the manufacturers' instructions.
|
| Data processing |
Images were analyzed using NimbleScan v2.5 software (Roche), background correction and standard RMA normalization were selected.
|
| |
|
| Submission date |
Nov 30, 2015 |
| Last update date |
Jan 10, 2017 |
| Contact name |
Marianna Fasoli |
| E-mail(s) |
[email protected]
|
| Phone |
+39 045 842 5625
|
| Organization name |
University of Verona
|
| Department |
Biotechnology
|
| Lab |
Molecular Viticulture
|
| Street address |
Via della Pieve 70
|
| City |
San Pietro in Cariano |
| State/province |
Verona |
| ZIP/Postal code |
37029 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17894 |
| Series (1) |
| GSE75498 |
Disclosing the Molecular Basis of the Postharvest Life of Berry in Different Grapevine Genotypes |
|
