|
| Status |
Public on May 26, 2009 |
| Title |
LowSES 5 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood CD2+ leukocytes
|
| Organism |
Homo sapiens |
| Characteristics |
other: Low socioeconomic status (SES)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Peripheral blood leukocytes were isolated from fresh whole blood by ficol density gradient, CD2+ cells were isolated by MACS immunomagnetic positive selection, and total RNA was extracted using Qiagen RNEasy.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix probe synthesis for gene expression profiling (carried out in UCLA DNA Microarray Core).
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol for gen expression profiling (carried out in UCLA DNA Microarray Core).
|
| Scan protocol |
Standard Affymetrixscan protocol for gene expression profiling (carried out in UCLA DNA Microarray Core).
|
| Description |
Affymetrix U133A
|
| Data processing |
RMA (RMAExpress) processing of Affymetrix .CEL files generated by UCLA DNA Microarray Core.
|
| |
|
| Submission date |
May 24, 2007 |
| Last update date |
Mar 29, 2010 |
| Contact name |
Steve Cole |
| Organization name |
UCLA School of Medicine
|
| Department |
Medicine
|
| Street address |
11-934 Factor Bldg, UCLA
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-1678 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7894 |
Differential gene expression in peripheral blood CD2+ leukocytes from PATH particpants |
|
