|
| Status |
Public on Mar 14, 2016 |
| Title |
d6TG-null-6 |
| Sample type |
RNA |
| |
|
| Source name |
TG cell cultures
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Prdm1-/-
cell type: trophoblast cells
|
| Growth protocol |
Trophoblast stem cells differentiated to trophoblast giant cells by withdrawal of FGF4, TGFbeta1 and heparin sulfate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN Rneasy mini kit and DNase I treatment on-column in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled according to the standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Prdm1 null d6 TG replicate 6
|
| Data processing |
Rank invariant normalization using Gene Expression Module V1.9.0 of Illumina GenomeStudio
|
| |
|
| Submission date |
Oct 27, 2015 |
| Last update date |
Mar 14, 2016 |
| Contact name |
Andrew Christopher Nelson |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Warwick
|
| Department |
School of Life Sciences
|
| Street address |
Gibbet Hill Campus
|
| City |
Coventry |
| ZIP/Postal code |
CV4 7AL |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE74409 |
Molecular function of Prdm1/Blimp1 in trophoblast giant cell differentiation. |
| GSE74410 |
Prdm1 |
|
