|
| Status |
Public on Sep 01, 2007 |
| Title |
expression at 16hr, bio rep2 |
| Sample type |
RNA |
| |
|
| Source name |
The RNA samples from DHT 16hr treated LNCaP cells
|
| Organism |
Homo sapiens |
| Characteristics |
LNCaP prostate cancer epithelial cell line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was first isolated using an RNeasy kit (Qiagen Catalog number 74104) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
RNA is converted into cDNA using a T7 promoter-tailed oligo-dT primer in the synthesis of the first cDNA strand; second strand cDNA synthesis is then carried out. The double-stranded cDNA is used as the template in an in vitro transcription (IVT) reaction catalyzed by T7 polymerase and containing biotinylated CTP and UTP in addition to the four unmodified ribonucleoside triphosphates. The biotinylated complementary RNA (cRNA) is purified from the IVT reaction mixture using the RNeasy system (Qiagen). The cRNA is quantified spectrophotometrically and purity of the cRNA is also assessed by spectrophometric measurements. Those cRNAs that fall outside of an acceptable range will not be carried forward in the analysis. The purified cRNA is fragmented in order to facilitate the subsequent hybridization step. The cRNA is purified from the fragmentation reaction using phenol/chloroform extraction and ethanol precipitation.
|
| |
|
| Hybridization protocol |
A35fluidics protocol EukGE-WS2v4_450 using Affymetrix fluidics Station 450
|
| Scan protocol |
Affymetrix GeneChip Scanner (GCS) 3000
|
| Description |
Gene expression data from LNCaP cell line treated with steroid hormone DHT
|
| Data processing |
creation of .EXP, .DAT, .CEL, .CHP, .RPT and .TXT files using GeneChip Operating System (GCOS) 1.4.0.036
|
| |
|
| Submission date |
May 22, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Wei Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Street address |
one baylor plaza, MS BCM305
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE7868 |
Expression data from LNCaP cell line |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
