|
| Status |
Public on Oct 10, 2016 |
| Title |
Medial geniculate nucleus of the thalamus, JJW022_2 |
| Sample type |
RNA |
| |
|
| Source name |
Thalamus, Df(16)1/+ wild type, 4 months
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: wild type
age: four months
tissue: medial geniculate nucleus of the thalamus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with mirVana isolation kit (Ambion). RNA quality was confirmed by analysis on the Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled with Cy3 according to Agilent's labeling protocol.
|
| |
|
| Hybridization protocol |
Microarray hybridization was performed at 55°C for 20 hours in Agilent's microarray hybridization chambers, followed by wash procedures according to the manufacturer's recommended protocols.
|
| Scan protocol |
Microarray was scanned in an Agilent scanner at 5μm resolution, and the array data was extracted with Agilent Feature Extraction software using the miRNA_105_Jan09 protocol.
|
| Description |
JJW022_2
Df(16)1/+ wild type, four months.
Biological replicate 3 of 4, technical replicate 2 of 2.
|
| Data processing |
Agilent Feature Extraction Software (version 10.5.1.1) was used for extracting the signal intensity, followed by a log2 transformation and quantile normalization between arrays among the entire dataset.
|
| |
|
| Submission date |
Oct 13, 2015 |
| Last update date |
Oct 11, 2016 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
[email protected]
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL17912 |
| Series (1) |
| GSE73981 |
miR-338-3p controls the late onset of auditory thalamocortical disruption in schizophrenia models |
|
