|
| Status |
Public on Oct 06, 2015 |
| Title |
LF33 |
| Sample type |
RNA |
| |
|
| Source name |
salivary gland_LF3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
cell type: salivary gland cancer stem cells
treated with: LF3 for 24 h
|
| Treatment protocol |
Cells were treated with LF3 and ICG-001 for 24 h
|
| Growth protocol |
Mouse salivary cancer stem cells were primarily isolated and cultured in in DMEM/F12 (Invitrogen) supplemented with 20% KSR, nonessential minimal amino acids, penicillin, streptomycin, L-glutamine and β-mercaptoethanol.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 9
|
| Data processing |
The data were normalised using quantile normalisation with Genomestudio (v.2010.1) with Modules Gene Expression 1.6.0 and Illumina Genome Viewer 1.6.1
|
| |
|
| Submission date |
Oct 05, 2015 |
| Last update date |
Oct 06, 2015 |
| Contact name |
Liang Fang |
| Organization name |
Max Delbrück Center for Molecular Medicine
|
| Lab |
Lab of Walter Birchmeier
|
| Street address |
Robert-Rössle-Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE73732 |
Gene expression profiling of mouse salivary gland cancer stem cells treated with Wnt inhibitors |
|
